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Genetic analysis of Pseudomonas aeruginosa isolates from patients with post-operative endophthalmitis

Pinna, Antonio <1963- > and Usai, Donatella and Sechi, Leonardo Antonio and Zanetti, Stefania Anna Lucia and Kaliamurthy, Jayaraman (2008) Genetic analysis of Pseudomonas aeruginosa isolates from patients with post-operative endophthalmitis. Acta Ophthalmologica, Vol. 86 (s243), 0-0. eISSN 1755-3768. Article.

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DOI: 10.1111/j.1755-3768.2008.682.x

Abstract

Purpose: To establish clonal relationship between strains of Pseudomonas aeruginosa isolated from 24 patients with postoperative endophthalmitis following cataract surgery Methods: All the patients with postoperative endophthalmitis were operated at the same operation theatre in the same hospital in Tiruchirapalli, Tamil Nadu, India. All the cases occurred within a time period of one month. Polymerase chain reaction (PCR) with enterobacterial repetitive intergenic consensus (ERIC) primers (ERIC-PCR) was used to establish clonal relationship between the different isolates. Results: ERIC-PCR disclosed 4 different patterns. Seventeen isolates showed an identical pattern, whereas 2 other strains were closely correlated with the former 17. Two other isolates, with identical ERIC-PCR pattern, were genetically similar, but to a lesser extent, to the first group. The remaining 3 isolates showed identical ERIC-PCR pattern, but they were less correlated with the other strains. Conclusion: The results of this study indicate that the outbreak of postoperative P. aeruginosa endophthalmitis presented here was caused predominantly by isolates with identical or very similar genetic patterns, thus suggesting a common source of infection in most cases. ERIC-PCR appears to be an inexpensive, fast, reproducible, and discriminatory DNA typing tool for effective epidemiological surveillance of outbreaks of P. aeruginosa eye infections.

Item Type:Article
ID Code:1715
Status:Published
Refereed:Yes
Uncontrolled Keywords:P. aeruginosa, Polymerase Chain Reaction (PCR), enterobacterial repetitive intergenic consensus (ERIC) primers (ERIC-PCR)
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Oftalmologia, Otorinolaringoiatria e Urologia
Publisher:Blackwell / Wiley
eISSN:1755-3768
Copyright Holders:Journal compilation © 2009 Acta Ophthalmologica
Additional Information:Abstracts in 2008 European Association for Vision and Eye Research Conference
Deposited On:18 Aug 2009 10:05

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