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Detection of Mycobacterium tuberculosis by PCR analysis of urine and other clinical samples from AIDS and non-HIV-infected patients

Sechi, Leonardo Antonio and Pinna, Maria Piera and Sanna, Alberto and Pirina, Pietro and Ginesu, Francesco and Saba, Franca and Aceti, Antonio and Turrini, Francesco Michelangelo and Zanetti, Stefania Anna Lucia and Fadda, Giovanni (1997) Detection of Mycobacterium tuberculosis by PCR analysis of urine and other clinical samples from AIDS and non-HIV-infected patients. Molecular and Cellular Probes, Vol. 11 (4), p. 281-285. ISSN 1096-1194. Article.

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DOI: 10.1006/mcpr.1997.0119

Abstract

A number of different clinical specimens, such as sputum, cerebrospinal fluid and blood, have been reported to be good substrates for the detection ofMycobacterium tuberculosisby PCR assay. We wanted to search for the presence of mycobacteria in other body fluids, such as urine. Urine samples and other samples obtained from AIDS patients and non HIV-infected patients were analysed by PCR. The results were compared with those obtained using conventional methods (Bactec 460 TB and AFB (acid fast bacilli strain)). We analysed 412 urine samples and 210 different other samples (sputum and cerebrospinal fluid) obtained from AIDS patients by PCR; almost identical levels of PCR-positive (14–17%) results were observed in all samples analysed. The results were then compared with those obtained with the Bactec 460 TB and AFB. PCR, Bactec 460 TB and acid fast stain were also used to analyse 190 urine samples and 230 other samples from non-HIV infected patients in the consumption ward of Sassari Hospital. The number of urine samples positive by PCR (6.3%) and Bactec 460 TB (2.1%) was half that obtained from samples taken from the AIDS patients. As expected, an increase in the number of positive sputum samples was observed with all methods. The results indicate that PCR analysis of urine samples represents a valid alternative for fast and sensitive detection ofM. tuberculosis. This method can be routinely used in the clinical laboratory, especially in HIV-infected patients.

Item Type:Article
ID Code:163
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium tuberculosis, nested-PCR, urine samples, AIDS patients
Subjects:Area 06 - Scienze mediche > MED/17 Malattie infettive
Area 06 - Scienze mediche > MED/10 Malattie dell'apparato respiratorio
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Nurex Bioresearch Sassari, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Tisiologia e malattie dell'apparato respiratorio
001 Università di Sassari > 03 Istituti > Malattie infettive e parassitarie
Publisher:Academic Press / Elsevier
ISSN:1096-1194
Deposited On:18 Aug 2009 10:01

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