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Cryopreservation of European mouflon (Ovis gmelini musimon) semen during the non-breeding season is enhanced by the use of trehalose

Berlinguer, Fiammetta and Leoni, Giovanni Giuseppe and Succu, Sara and Mossa, Francesca and Galioto, Manuela and Madeddu, Manuela and Naitana, Salvatore (2007) Cryopreservation of European mouflon (Ovis gmelini musimon) semen during the non-breeding season is enhanced by the use of trehalose. Reproduction in Domestic Animals, Vol. 42 (2), p. 202-207. eISSN 1439-0531. Article.

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DOI: 10.1111/j.1439-0531.2006.00753.x

Abstract

The influence of trehalose on European mouflon spermatozoa cryopreservation during the non-breeding season was tested. Semen was frozen in two different extenders: (a) recommended Tris-based ram extender (CTR); (b) CTR extender supplemented with trehalose 0.147 mM (TRH). Sperm viability and acrosome integrity were assessed using propidium iodide and fluorescein isothiocynate labelled Pisum Sativum agglutinin. Trehalose significantly enhanced sperm viability after thawing compared with CTR extender (62.7% vs 51.8%; p < 0.05), whereas no differences were observed on acrosome integrity (42.9% vs 42.1%). Trehalose influence was also evidenced in the in vitro fertility test performed with sheep oocytes matured in vitro. Both fertilization rates (60.9% TRH vs 43.6% CTR; p < 0.05) and cleavage rates (58% TRH vs 39.8% CTR; p < 0.001) were higher for trehalose frozen semen compared with control extender frozen semen. A higher percentage of zygotes resulting from fertilization with trehalose cryopreserved semen presented the first cleavage earlier if compared with the group fertilized with control semen (48.7% vs 31.5%, respectively; p < 0.01). This result was confirmed by embryo kinetic development. Fertilization with trehalose cryopreserved semen leaded to an higher percentage of blastocysts (40.2% vs 27.8% CTR; p < 0.05), and enhanced in particular the number of blastocysts that developed on the day 6th of culture (28.6% vs 17% CTR; p < 0.05). Our data demonstrated that, during mouflon non-breeding season, trehalose extender enhances spermatozoa viability and its in vitro fertilizing capacity, allowing the production of an higher number of blastocysts.

Item Type:Article
ID Code:1605
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mouflon spermatozoa, trehalose
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
eISSN:1439-0531
Copyright Holders:© 2007 The Authors, Journal compilation © 2007 Blackwell
Deposited On:18 Aug 2009 10:05

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