Rao, Donepudi Venkateswara and Swapna, Medasani and Cesareo, Roberto and Brunetti, Antonio and Akatsuka, Takao and Yuasa, Tetsuya and Takeda, Tohoru and Tromba, Giuliana and Gigante, Giovanni Ettore (2009) Synchrotron-induced X-ray fluorescence from rat bone and lumber vertebra of different age groups. Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, Vol. 267 (3), p. 502-505. ISSN 0168-583X. Article.
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The fluorescence spectra from rat bones of different age groups (8, 56 and 78 weeks) and lumber vertebra were measured with 8, 10 and 12 keV synchrotron X-rays. We have utilized the new hard X-ray micro-spectroscopy beamline facility, X27A, available at NSLS with a primary beam spot size of the order of ~10 μm. With this spatial resolution and high flux throughput, X-ray fluorescent intensities for Ca and other trace elements were measured using a liquid-nitrogen-cooled 13-element energy-dispersive high-purity germanium detector. Regarding the lumber vertebra, we acquired the fluorescence spectra from the left, right and middle portions and calcium accumulation was evaluated and compared with the other samples. We have identified the major trace elements of Ca, Ni, Fe and Zn and minor trace elements of Ti, Cr and Mn in the sample. The percentage of scattered radiation and trace element contributions from these samples were highlighted at different energies.
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