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A Direct RP-HPLC method for the determination of furanic aldehydes and acids in honey

Spano, Nadia and Ciulu, Marco and Floris, Ignazio and Panzanelli, Angelo and Pilo, Maria Itria and Piu, Paola Costantina and Salis, Severyn and Sanna, Gavino (2009) A Direct RP-HPLC method for the determination of furanic aldehydes and acids in honey. Talanta, Vol. 78 (1), p. 310-314. ISSN 0039-9140. Article.

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DOI: 10.1016/j.talanta.2008.11.015


In this study 5-hydroxymethyl-2-furaldehyde (HMF), 2-furaldehyde, 3-furaldehyde, 2-furoic acid and 3-furoic acid are contemporarily determined in honey using a swift and direct RP-HPLC approach. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and reproducibility), linearity and accuracy (by recovery tests); the acceptability of the precision and accuracy results was positively verified using Horwitz's model and AOAC guidelines, respectively. The method was tested on 18 honey samples of different ages, and botanical and geographical origin. HMF and 2-furaldehyde correlated highly with the age of the samples, whereas no correlation was observed with regards to 2-furaldehyde and 2-furoic acid. Hypotheses relating to the formation of minority furanic compounds are also proposed.

Item Type:Article
ID Code:1519
Uncontrolled Keywords:Honey, 5-hydroxymethyl-2-furaldehyde (HMF), furfural, 2-furaldehyde, 3-furaldehyde, 2-furoic acid, 3-furoic acid
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/11 Entomologia generale e applicata
Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
001 Università di Sassari > 01 Dipartimenti > Chimica
Deposited On:18 Aug 2009 10:05

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