Desantis, Salvatore and Ventriglia, Gianluca and Zubani, Dolores and Deflorio, Michele and Megalofonou, Persefoni and Acone, Franca and Zarrilli, Antonia and Palmieri, Giovanni and De Metrio, Gregorio (2006) Histochemical analysis of glycoconjugates in the domestic cat testis. Histology and Histopathology, Vol. 21 (1), p. 11-22. eISSN 1699-5848. Article.
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The localization and characterization of oligosaccharide sequences in the cat testis was investigated using 12 lectins in combination with the ß-elimination reaction, N-Glycosidase F and sialidase digestion. Leydig cells expressed O-linked glycans with terminal αGalNAc (HPA reactivity) and N-glycans with terminal/internal aMan (Con A affinity). The basement membrane showed terminal Neu5Acα2,6Gal/GalNAc, Galß1,3GalNAc, α/ßGalNAc, and GlcNAc (SNA, PNA, HPA, SBA, GSA II reactivity) in O-linked oligo-saccharides, terminal Galß1,4GlcNAc (RCA120 staining) and αMan in N-linked oligosaccharides; in addition, terminal Neu5acα2,3Galß1,4GlcNac, Forssman pentasaccharide, αGal, αL-Fuc and internal GlcNAc (MAL II, DBA, GSA I-B4, UEA I, KOH-sialidase-WGA affinity) formed both O- and N-linked oligosaccharides. The Sertoli cells cytoplasm contained terminal Neu5Ac-Galß1,4GlcNAc, Neu5Ac-ßGalNAc as well as internal GlcNAc in O-linked glycans, αMan in N-linked glycoproteins and terminal Neu5Acα2,6Gal/GalNAc in both O- and N-linked oligosaccharides. Spermatogonia exhibited cytoplasmic N-linked glycoproteins with aMan residues. The spermatocytes cytoplasm expressed terminal Neu5acα2,3Galß1,4GlcNAc and Galß1,3GalNAc in O-linked oligo-saccharides, terminal Galß1,4GlcNAc and α/ßGalNAc in N-linked glycoconjugates. The Golgi region showed terminal Neu5acα2,3Galß1,4GlcNac, Galß1,4GlcNAc, Forssman pentasaccharide, and αGalNAc in O-linked oligosaccharides, αMan and terminal ßGal in N-linked oligosaccharides. The acrosomes of Golgi-phase spermatids expressed terminal Galß1,3GalNAc, Galß1,4GlcNAc, Forssmann pentasaccharide, α/ßGalNAc, αGal and internal GlcNAc in O-linked oligosaccharides, terminal α/ßGalNAc, αGal and terminal/internal αMan in N-linked glycoproteins. The acrosomes of cap-phase spermatids lacked internal Forssman pentasaccharide and αGal, while having increased α/ßGalNAc. The acrosomes of elongated spermatids did not show terminal Galß1,3GalNAc, displayed terminal Galß1,4GlcNAc and α/ßGalNAc in N-glycans and Neu5Ac-Galß1,3GalNAc in O-linked oligosaccharides.
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