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Embryonic stem cell differentiation studied by FT-IR spectroscopy

Ami, Diletta and Neri, Tui and Natalello, Antonino and Mereghetti, Paolo and Doglia, Silvia Maria and Zanoni, Mario and Zuccotti, Maurizio and Garagna, Silvia and Redi, Carlo Alberto (2008) Embryonic stem cell differentiation studied by FT-IR spectroscopy. Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1783 (1), p. 98-106. ISSN 0167-4889. Article.

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DOI: 10.1016/j.bbamcr.2007.08.003

Abstract

We propose, here, an FT-IR method to monitor the spontaneous differentiation of murine embryonic stem (ES) cells in their early development. Principal component analysis and subsequent linear discriminant analysis enabled us to segregate stem cell spectra into separate clusters – corresponding to different differentiation times – and to identify the most significant spectral changes during differentiation. Between days 4 to 7 of differentiation, these spectral changes in the protein amide I band (1700–1600 cm− 1) and in the nucleic acid absorption region (1050–850 cm− 1) indicated that mRNA translation was taking place and that specific proteins were produced, reflecting the appearance of a new phenotype. The DNA/RNA hybrid bands (954 cm− 1 and 899 cm− 1) were also observed, suggesting that the transcriptional switch of the genome started at this stage of differentiation. As confirmed by cytochemical assays, the FT-IR approach presented here allows to detect at molecular level the biological events of ES cell differentiation as they take place and to monitor in a rapid way the temporal evolution of the ES cell culture.

Item Type:Article
ID Code:1448
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cardiomyocyte, cytodifferentiation, embryonic stem cell, fourier transform infrared spectroscopy, linear discriminant analysis, Principal component analysis
Subjects:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:Elsevier Science
ISSN:0167-4889
Additional Information:Pubblicato online il 28 agosto 2007.
Deposited On:18 Aug 2009 10:05

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