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Enantiomeric reversed-phase high-performance liquid chromatography resolution of d-/l-penicillamine after spirocyclization with ninhydrin and by using copper(II)-l-proline complex as a chiral selector in the mobile phase

Sotgia, Salvatore and Zinellu, Angelo and Pisanu, Elisabetta and Pinna, Gérard Aimé and Deiana, Luca and Carru, Ciriaco (2008) Enantiomeric reversed-phase high-performance liquid chromatography resolution of d-/l-penicillamine after spirocyclization with ninhydrin and by using copper(II)-l-proline complex as a chiral selector in the mobile phase. Journal of Chromatography A, Vol. 1205 (1-2), p. 90-93. ISSN 0021-9673. Article.

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DOI: 10.1016/j.chroma.2008.08.021

Abstract

A new HPLC method by fluorescence or UV/vis absorbance detection has been developed for the separation and quantification of penicillamine stereoisomers after their spirocyclization with ninhydrin. The separation was performed on an achiral C18 column by isocratic elution with a copper(II)-l-proline complex as a chiral selector in the mobile phase. The method was able to detect traces of l-penicillamine in samples of d-penicillamine below 0.1% in fairly short times (about 16 min) with a good resolution (Rs = 1.31). On the whole, the method was found to be stable and useful in the quality control of the bulk material and formulations.

Item Type:Article
ID Code:1383
Status:Published
Refereed:Yes
Uncontrolled Keywords:Derivatization, penicillamine, ninhydrin, chiral ligand-exchange chromatography, spirothiazolidine, chirality
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
Publisher:Elsevier Science
ISSN:0021-9673
Deposited On:18 Aug 2009 10:05

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