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Relationship between MTNR1A melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds

Carcangiu, Vincenzo and Vacca, Giuseppe Massimo and Mura, Maria Consuelo and Dettori, Maria Luisa and Pazzola, Michele and Luridiana, Sebastiano and Bini, Pier Paolo (2009) Relationship between MTNR1A melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds. Animal Reproduction Science, Vol. 110 (1-2), p. 71-78. ISSN 0378-4320. Article.

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DOI: 10.1016/j.anireprosci.2007.12.014

Abstract

The reproductive activity of goats bred in temperate latitude follows a seasonal pattern, influenced by annual variation in day length. Daily variation in pineal melatonin secretion is the neuroendocrine signal recognized by animals through the link between this hormone and melatonin receptor 1a (MTNR1A). A total of 345 goats of different breeds (225 Sarda, 30 Saanen, 30 Chamois Coloured, 30 Maltese and 30 Nubian) with a kidding period in October–December or January–March were analysed to verify if a link exists between the structure of the receptor gene and reproductive activity. The main part of exon II of MTNR1A gene was amplified by PCR and then digested with MnlI and RsaI to prove the presence of restriction sites. Sequencing of 20 cloned samples and 20 purified samples permitted comparison with previously published sequences. No polymorphism was found using MnlI enzyme, as all 345 samples showed the cleavage site in position 605 and all the goats were MM genotype. However, using RsaI enzyme, some Sarda goats, showed a polymorphic site in position 53. Nine Sarda goats were R/r genotype, lacking this cleavage site only in one allele, while the other animals, both Sarda and the other breeds, presented the cleavage site in both the alleles and were thus R/R genotype. No r/r genotype was found in any of the breeds. In Sarda goats the allelic frequency was 0.98 for R allele and 0.02 for r allele; genotypic frequency was 96.00% for R/R genotype and 4.00% for R/r genotype. A strong link emerged from statistical analysis (P < 0.001) between R/r genotype and reproductive activity, which was strongly influenced by photoperiod. Sequencing indicated six nucleotide changes that did not induce any amino acid change. Data showed that polymorphism was present and that it influences reproductive activity only in the Sarda breed.

Item Type:Article
ID Code:1381
Status:Published
Refereed:Yes
Uncontrolled Keywords:Goat, melatonin receptor gene, seasonal reproduction
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 07 - Scienze agrarie e veterinarie > AGR/17 Zootecnica generale e miglioramento genetico
Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
ISSN:0378-4320
Deposited On:18 Aug 2009 10:05

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