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Use of a neuroleptic in assisted reproduction of the critically endangered Mohor gazelle (Gazella dama mhorr)

González, Raquel and Berlinguer, Fiammetta and Espeso Pajares, Gerardo and Ariu, Federica and Del Olmo, Ana and Garde Lopez-Brea, Jose Julian and Gomendio Kindelan, Montserrat and Ledda, Sergio and Roldan, Eduardo R. S. (2008) Use of a neuroleptic in assisted reproduction of the critically endangered Mohor gazelle (Gazella dama mhorr). Theriogenology, Vol. 70 (6), p. 909-922. ISSN 0093-691X. Article.

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DOI: 10.1016/j.theriogenology.2008.05.052

Abstract

Stress is a limiting factor in assisted reproduction in wild animals maintained in captivity and measures to reduce it should improve reproductive success. The effect of the long-acting neuroleptic (LAN) perphenazine enanthate was assessed on ovarian stimulation for the recovery of immature oocytes from Mohor gazelle (Gazella dama mhorr) and their subsequent in vitro maturation, fertilization and embryo culture. The viability of embryos after transfer was also examined. Perphenazine enanthate decreased activity levels and facilitated handling of treated animals when compared to controls. LAN-treated animals showed a more regular pattern of respiratory and heart rates and body temperature than controls; no major differences were found in hematological and biochemical parameters between groups. Perphenazine-treated females had lower plasma cortisol levels during the days of intense handling. No significant differences were found in the number of punctured follicles and recovered oocytes between groups. The percentage of mature oocytes per female was significantly higher in the LAN-group. Fertilization and cleavage rates were not significantly different between groups. Embryos developed in culture but none reached the blastocyst stage, and those transferred to the oviduct of synchronized recipients did not develop to term. In conclusion, treatment of females with perphenazine enanthate during ovarian stimulation did not have negative effects on maturation, fertilization and embryo development in vitro. Moreover, an increase in oocyte maturation rate per female was observed. Thus, the use of LANs could be useful to alleviate the effects of handling-stress during assisted reproductive procedures in wild ungulates.

Item Type:Article
ID Code:1380
Status:Published
Refereed:Yes
Uncontrolled Keywords:Oocyte, fertilization, stress, cortisol, long-acting neuroleptic
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Divisions:001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
ISSN:0093-691X
Deposited On:18 Aug 2009 10:05

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