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Microsatellite instability and mutation analysis of candidate genes in unselected Sardinian patients with endometrial carcinoma

Baldinu, Paola and Cossu, Antonio and Manca, Antonella and Satta, Maria Paola and Pisano, Marina and Casula, Milena and Dessole, Salvatore and Pintus, Adriana and Tanda, Francesco and Palmieri, Giuseppe (2002) Microsatellite instability and mutation analysis of candidate genes in unselected Sardinian patients with endometrial carcinoma. Cancer, Vol. 94 (12), p. 3157-3168. eISSN 1097-0142. Article.

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DOI: 10.1002/cncr.10606

Abstract

BACKGROUND. Microsatellite instability (MSI) is due mostly to a defective DNA mismatch repair (MMR). Inactivation of the two principal MMR genes, hMSH1and hMSH2, and the PTEN tumor suppressor gene seems to be involved in endometrial tumorigenesis. In this study, Sardinian patients with endometrial carcinoma (EC) were analyzed to assess the prevalence of both the mutator phenotype (as defined by the presence of MSI and abnormal MMR gene expression at the somatic level) and the hMSH2, hMSH1, and PTEN germline mutations among patients with MSI positive EC. METHODS. Paraffin embedded tissue samples from 116 consecutive patients with EC were screened for MSI by polymerase chain reaction-based microsatellite analysis. Immunohistochemistry (IHC) with anti-hMLH1 and anti-hMSH2 antibodies was performed on MSI positive tumor tissue sections. Germline DNA was used for mutational screening by denaturing high-performance liquid chromatography analysis and automated sequencing. RESULTS. Thirty-nine patients with EC (34%) exhibited MSI; among them, 25 tumor samples (64%) showed negative immunostaining for hMLH1/hMSH2 proteins (referred to as IHC negative). No disease-causing mutation within the coding sequences of the hMSH1/hMSH2 and PTEN genes was found in patients with EC who had the mutator phenotype (MSI positive and IHC negative), except for a newly described hMSH1 missense mutation, Ile655Val, that was observed in 1 of 27 patients (4%). Although MSI was more common among patients with advancedstage EC and increased as the tumor grade increased, no significant correlation with disease free survival or overall survival was observed among the two groups (MSI positive or MSI negative) of patients with EC. CONCLUSIONS. In patients with MSI positive EC, epigenetic inactivations rather than genetic mutations of the MMR genes seem to be involved in endometrial tumorigenesis. No prognostic value was demonstrated for MSI in patients with EC.

Item Type:Article
ID Code:1357
Status:Published
Refereed:Yes
Uncontrolled Keywords:Endometrial carcinoma, polymerase chain reaction, microsatellite instability, immunohistochemistry, hMLH1, hMSH2, PTEN, mutational analysis, prognosis
Subjects:Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
Area 06 - Scienze mediche > MED/08 Anatomia patologica
Divisions:001 Università di Sassari > 03 Istituti > Anatomia patologica
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di genetica delle popolazioni, Alghero
Publisher:Wiley
eISSN:1097-0142
Copyright Holders:© 2002 American Cancer Society
Deposited On:18 Aug 2009 10:04

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