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The Mesolimbic dopaminergic response to novel palatable food consumption increases dopamine-D1 receptor-mediated signalling with complex modifications of the DARPP-32 phosphorylation pattern

Rauggi, Riccardo and Scheggi, Simona and Cassanelli, Anna and De Montis, Maria Graziella and Tagliamonte, Alessandro and Gambarana, Carla (2005) The Mesolimbic dopaminergic response to novel palatable food consumption increases dopamine-D1 receptor-mediated signalling with complex modifications of the DARPP-32 phosphorylation pattern. Journal of Neurochemistry, Vol. 92 (4), p. 867-877. eISSN 1471-4159. Article.

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DOI: 10.1111/j.1471-4159.2004.02920.x

Abstract

Acute cocaine administration increases extraneuronal dopamine and Thr34 phosphorylation of dopamine- and cAMP-regulated phosphoprotein (Mr 32 kDa; DARPP-32) in striatal and cortical areas. Novel palatable food consumption increases extraneuronal dopamine in the same areas. We examined the DARPP-32 phosphorylation pattern in food non-deprived rats at different times after vanilla sugar consumption. The phosphorylation state of DARPP-32 and two cAMP-dependent protein kinase (PKA) substrates, GluR1 and NR1, were detected by immunoblotting. Thirty to 45 min after vanilla sugar consumption, phospho-Thr34 DARPP-32, GluR1 and NR1 levels increased in the nucleus accumbens, and phospho-Thr75 DARPP-32 levels decreased. At 60 min, all parameters returned to baseline values. However, 2 and 3 h after vanilla sugar consumption, phospho-Thr34 DARPP-32 levels decreased, while phospho-Thr75 DARPP-32 levels increased. In contrast to the pattern observed in the NAcS, no delayed changes in DARPP-32 phosphorylation were observed in the mPFC. Both early and delayed DARPP-32, GluR1 and NR1 phosphorylation changes were prevented by a dopamine D1 receptor antagonist administration. The delayed modifications in nucleus accumbens DARPP-32 phosphorylation were prevented by an mGluR5 antagonist administration. The mesolimbic dopaminergic response to an unfamiliar taste is correlated to a gustatory memory trace development, and the observed changes in DARPP-32 phosphorylation may be part of this process.

Item Type:Article
ID Code:1353
Status:Published
Refereed:Yes
Uncontrolled Keywords:Medial pre-frontal cortex, metabotropic glutamatergic receptors, microdialysis, nucleus accumbens, rat, western blotting
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:Blackwell / Wiley
eISSN:1471-4159
Copyright Holders:© 2005 International Society for Neurochemistry
Additional Information:Pubblicato online il 24 gennaio 2005.
Deposited On:18 Aug 2009 10:04

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