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Random amplified polymorphic DNA and amplified ribosomal DNA spacer polymorphism: powerful methods to differentiate Streptococcus thermophilus strains

Moschetti, Giancarlo and Blaiotta, Giuseppe and Aponte, Maria and Catzeddu, Pasquale and Villani, Francesco and Deiana, Pietrino and Coppola, Salvatore (1998) Random amplified polymorphic DNA and amplified ribosomal DNA spacer polymorphism: powerful methods to differentiate Streptococcus thermophilus strains. Journal of Applied Microbiology, Vol. 90 (2), p. 25-36. eISSN 1365-2672. Article.

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DOI: 10.1046/j.1365-2672.1998.00461.x

Abstract

Streptococci from different collections and dairy materials were characterized by conventional and molecular methods. After amplification of the 16S–23S rDNA spacer region, all the strains referable to the genus Streptococcus exhibited a single polymerase chain reaction (PCR) product, allowing their differentiation from enterococci. Cleaving this PCR product with Hae III, two different restriction patterns could be observed, allowing Streptococcus salivarius DSM 20560T, Strep. thermophilus NCDO 822 and two strains of Streptococcus spp. to be gathered in one group and all the other strains in another. In order to achieve strain typing, all the cultures were investigated by random amplified polymorphic DNA (RAPD)-PCR analysis employing two selected primers. The results were treated by cluster analysis, appearing significantly consistent with both the taxonomic position and the origin of the strains. Pulsed-field gel electrophoresis (PFGE) of Sma I digests of the genomic DNA from 11 representative strains with decreasing levels of RAPD similarity allowed their diversity to be confirmed, even though RAPD-PCR proved to be less discriminating than PFGE analysis. The results are discussed with reference to the capability of the analytical procedures used to aid both identification and strain typing of streptococci, as well as the taxonomic structure of the species Strep. thermophilus.

Item Type:Article
ID Code:1344
Status:Published
Refereed:Yes
Uncontrolled Keywords:Streptococcus thermophilus, thermophilic starters, fermented dairy products, PCR protocols
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:The Society for Applied Microbiology
eISSN:1365-2672
Additional Information:Pubblicato online il 5 gennaio 2002.
Deposited On:18 Aug 2009 10:04

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