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Reactions of gold(III) complexes with serum albumin

Marcon, Giordana and Messori, Luigi and Orioli, Pierluigi and Cinellu, Maria Agostina and Minghetti, Giovanni (2003) Reactions of gold(III) complexes with serum albumin. European Journal of Biochemistry, Vol. 270 (23), p. 4655-4661. eISSN 1432-1033. Article.

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DOI: 10.1046/j.1432-1033.2003.03862.x

Abstract

The reactions of a few representative gold(III) complexes –[Au(ethylenediamine)2]Cl3, [Au(diethylentriamine)Cl]Cl2, [Au(1,4,8,11-tetraazacyclotetradecane)](ClO4)2Cl, [Au(2,2',2'-terpyridine)Cl]Cl2, [Au(2,2'-bipyridine)(OH)2][PF6] and the organometallic compound [Au(6-(1,1-dimethylbenzyl)-2,2'-bipyridine-H)(OH)][PF6]– with BSA were investigated by the joint use of various spectroscopic methods and separation techniques. Weak metal–protein interactions were revealed for the [Au(ethylenediamine)2]3+ and [Au(1,4,8,11-tetraazacyclotetradecane)]3+ species, whereas progressive reduction of the gold(III) centre was observed in the cases of [Au(2,2'-bipyridine)(OH)2]+ and [Au(2,2',2'-terpyridine)Cl]2+. In contrast, tight metal–protein adducts are formed when BSA is reacted with either [Au(diethylentriamine)Cl]2+ and [Au(6-(1,1-dimethylbenzyl)-2,2'-bipyridine-H)(OH)]+. Notably, binding of the latter complex to serum albumin results in the appearance of characteristic CD bands in the visible spectrum. It is suggested that adduct formation for both of these gold(III) complexes occurs through coordination at the level of surface histidines. Stability of these gold(III) complexes/serum albumin adducts was tested under physiologically relevant conditions and found to be appreciable. Metal binding to the protein is tight; complete detachment of the metal from the protein has been achieved only after the addition of excess potassium cyanide. The implications of the present results for the pharmacological activity of these novel cytotoxic agents are discussed.

Item Type:Article
ID Code:1285
Status:Published
Refereed:Yes
Uncontrolled Keywords:Gold(III) complexes, serum albumin, spectroscopic measurements
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:Blackwell Science on behalf of the Federation of European Biochemical Societies / Wiley
eISSN:1432-1033
Copyright Holders:© FEBS 2003
Deposited On:18 Aug 2009 10:04

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