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Thermal stability of lysozyme Langmuir-Schaefer films by FTIR spectroscopy

Pechkova, Eugenia and Innocenzi, Plinio and Malfatti, Luca and Kidchob, Tongjit and Gaspa, Leonardo and Nicolini, Claudio (2007) Thermal stability of lysozyme Langmuir-Schaefer films by FTIR spectroscopy. Langmuir, Vol. 23 (3), p. 1147-1151. eISSN 1520-5827. Article.

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DOI: 10.1021/la061970o

Abstract

Fourier transform infrared spectroscopy has been applied to study the thermal stability of multilayer Langmuir- Schaefer (LS) films of lysozyme deposited on silicon substrates. The study has confirmed previous structural findings that the LS protein films have a high thermal stability that is extended in a lysozyme multilayer up to 200 °C. 2D infrared analysis has been used here to identify the correlated molecular species during thermal denaturation. Asynchronous 2D spectra have shown that the two components of water, fully and not fully hydrogen bonded, in the high-wavenumber range (2800-3600 cm-1) are negatively correlated with the amine stretching band at 3300 cm-1. On the grounds of the 2D spectra the FTIR spectra have been deconvoluted using three main components, two for water and one for the amine. This analysis has shown that, at the first drying stage, up to 100 °C, only the water that is not fully hydrogen bonded is removed. Moreover, the amine intensity band does not change up to 200 °C, the temperature at which the structural stability of the multilayer lysozyme films ceases.

Item Type:Article
ID Code:1257
Status:Published
Refereed:Yes
Uncontrolled Keywords:Langmuir−Schaefer (LS) films, thermal stability, FTIR spectroscopy (Fourier Transform Infrared)
Subjects:Area 09 - Ingegneria industriale e dell'informazione > ING-IND/22 Scienza e tecnologia dei materiali
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > INSTM-Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali, Unità di ricerca di Sassari
001 Università di Sassari > 01 Dipartimenti > Architettura e pianificazione
Publisher:American Chemical Society
eISSN:1520-5827
Additional Information:Pubblicato online il 12 settembre 2006.
Deposited On:18 Aug 2009 10:04

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