Wojtowicz, Andrzej and Yamauchi, Mitsuo and Montella, Andrea Costantino Mario and Bandiera, Pasquale and Sotowski, R. and Ostrowski, Kazimierz (1999) Persistence of bone collagen cross-links in skeletons of the nuraghi population. Calcified Tissue International, Vol. 64 (5), p. 370-373. eISSN 1432-0827. Article.
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Bone collagen has a specific molecular ultrastructure which can be proved by birefringence. This protein, forming the main organic component of bone tissue, is known to survive millennia in paleontological bones and teeth. Birefringence of bone collagen obtained from the skeletons of the Nuraghi population living in Sardinia c-ca 1500 years B.C. was found previously by the use of polarizing microscopy . In this paper, using high pressure liquid chromatography (HPLC) techniques, we show the existence of bone collagen cross-links preserved in Nuraghi skeletons after more than 3000 years.
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