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Implication of Bcl-2 family genes in basal and d-amphetamine-induced apoptosis in preneoplastic and neoplastic rat liver lesions

De Miglio, Maria Rosaria and Muroni, Maria Rosaria and Simile, Maria Maddalena and Calvisi, Diego Francesco and Tolu, Paola and Deiana, Luca and Carru, Angelo and Bonelli, Gabriella and Feo, Francesco and Pascale, Rosa Maria (2000) Implication of Bcl-2 family genes in basal and d-amphetamine-induced apoptosis in preneoplastic and neoplastic rat liver lesions. Hepatology, Vol. 31 (4), p. 956-965. eISSN 1527-3350. Article.

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DOI: 10.1053/he.2000.5411


Molecular mechanisms of basal and D-amphetamine (AMPH)-induced apoptosis were studied in rat liver nodules, 12 (N12) and 30 (N30) weeks after initiation, and in hepatocellular carcinoma (HCC) induced by diethylnitrosamine in rats subjected to resistant hepatocyte model. Basal apoptosis in hematoxylin/eosin– and propidium iodide– stained sections was higher in nodules and HCC than in normal livers. It sharply increased in all tissues 4 hours after AMPH treatment (10 mg/kg), and declined to basal levels at 8 to 12 hours in liver and N12, but remained high up to 18 hours in N30 and HCC. c-myc, Tgf- α, p53, and Bcl-XS messenger RNA (mRNA) levels were higher, and Bcl-2 mRNA was lower in N12 and/or N30 and HCC than in normal liver. Four hours after AMPH injection, increase in c-myc and decreases in Bcl-2 and Bcl-XL mRNAs occurred in all tissues, whereas p53, Bax, and Bcl-XS mRNAs increased in N30 and HCC. These changes disappeared in liver and N12 at 18 hours, but persisted in N30 and HCC. c-Myc, P53, Bcl-2, and Bax proteins in normal liver and HCC 6 AMPH showed similar patterns. Tgf- β 1, Tgf- β-RIII, CD95, and CD95L mRNA levels underwent slight or no changes in any tissue 6 AMPH. Basal Hsp27 expression was high in nodules and HCC, and was stimulated by AMPH in liver and N12, but not in N30 and HCC. These data suggest a role of dysregulation of Bcl-2 family genes and, at least in atypical lesions, of p53 overexpression, in basal and AMPHinduced apoptosis in nodules and HCCs. Hsp27 does not appear to sufficiently protect atypical lesions against apoptosis.(HEPATOLOGY 2000;31:956-965.)

Item Type:Article
ID Code:1224
Uncontrolled Keywords:Hepatocellular carcinoma (HCC), diethylnitrosamine, mouse model
Subjects:Area 06 - Scienze mediche > MED/04 Patologia generale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Copyright Holders:© 2000 by the American Association for the Study of Liver Diseases
Additional Information:Pubblicato online il 30 dicembre 2003.
Deposited On:18 Aug 2009 10:04

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