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Temperature dependence of chaperone-like activity and oligomeric state of alphaB-crystallin

Spinozzi, Francesco and Mariani, Paolo and Rustichelli, Franco and Heinz, Amenitsch and Bennardini, Federico and Mura, Giovanni Maria and Coi, Alessio and Ganadu, Maria Luisa Margherita (2006) Temperature dependence of chaperone-like activity and oligomeric state of alphaB-crystallin. Biochimica et Biophysica Acta. Proteins & Proteomics, Vol. 1764 (4), p. 677-687. ISSN 0304-4165. Article.

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DOI: 10.1016/j.bbapap.2006.02.003

Abstract

The chaperone-like activity and the oligomeric state of αB-crystallin were studied at different temperatures and in the presence of urea and thiocyanate. The activity, assessed measuring the ability of αB-crystallin to prevent the aggregation of denatured insulin, strongly depends on temperature. While a significant activity increase was detected at 42°C, the presence of urea and thiocyanate does not affect the protein activity in an irreversible way. In-solution SAXS measurements performed in the same experimental conditions showed that αB-crystallin forms near-spherical, hollowed, polydisperse oligomers, whose dimensions change above 42°C. Moreover, in the presence of urea and thiocyanate, a global fit analysis confirms the high stability of αB-crystallin assemblies in relationship with their variable quaternary structure. In particular, the changes in the inner radius as well as the thickness and dispersion of the protein shell, account for the preservation of the chaperone-like activity.

Item Type:Article
ID Code:1205
Status:Published
Refereed:Yes
Uncontrolled Keywords:αB-crystallin, SAXS, chaperone activity, heat shock protein, protein
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 03 - Scienze chimiche > CHIM/12 Chimica dell'ambiente e dei beni culturali
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:Elsevier Science
ISSN:0304-4165
Deposited On:18 Aug 2009 10:04

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