Tamponi, Claudia (2019) Aggiornamenti sulle infezioni da protozoi negli ovini della Sardegna. Doctoral Thesis.
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The aim of the present study was to deepened knowledge on epidemiology and taxonomy of Cryptosporidiosis, Sarcocystosis and Giardiosis in Sardinian dairy sheep.
Cryptosporidiosis was evaluated with microscopical and biomolecular methods in faecal samples from three different categories of sheep (lambs, pregnant sheep, post-partum sheep). Oocysts were found in 10.1% of examined animals, in 16.4% of lambs, in 7.2% of pregnant sheep and in 6.6% of post-partum sheep. Molecular characterization revealed the presence of two different species, Cryptosporidium parvum and C. ubiquitum. Within C. parvum were found the subtypes IIaA15G2R1 and IIdA20G1 while the subtype XIIa was isolated for C. ubiquitum.
Giardiosis was evaluated in the same sheep population, and Giardia cyst were microscopically detected in 6.6% of monitored farms, only in lambs. Molecular characterization of samples allow to identify the Assemblage AI of Giardia duodenalis.
Sarcosporidiosis was investigated with a cross sectional study on macroscopic and microscopic sarcocysts in Sardinian sheep at abattoirs. Macroscopic Sarcocystis spp. were found in 23.3% of examined muscles. Two different morphotypes were identified: large oval cysts, identified as Sarcocystis gigantea by molecular characterization, and slender fusiform cysts, identified as S. medusiformis. Microscopic cysts were detected in 77.7% of examined heart samples, molecular analysis allow to identify the species S. tenella (95.5%) and S. arieticanis (17.8%).
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