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Physicochemical, antioxidant, DNA cleaving properties and antimicrobial activity of fisetin-copper chelates

Łodyga-Chruscińska, Elżbieta and Pilo, Maria Itria and Zucca, Antonio and Garribba, Eugenio and Klewicka, Elżbieta and Symonowicz, Marzena and Chrusciński, Longin and Cheshchevik, Vitalij T. (2018) Physicochemical, antioxidant, DNA cleaving properties and antimicrobial activity of fisetin-copper chelates. Journal of Inorganic Biochemistry, Vol. 180 , p. 101-118. ISSN 0162-0134. Article.

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DOI: 10.1016/j.jinorgbio.2017.12.006

Abstract

Fisetin (3,3′,4′,7-tetrahydroxyflavone) metal chelates are of interest as this plant polyphenol has revealed broad prospects for its use as natural medicine in the treatment of various diseases. Metal interactions may change or enhance fisetin biological properties so understanding fisetin metal chelation is important for its application not only in medicine but also as a food additive in nutritional supplements. This work was aimed to determine and characterize copper complexes formed in different pH range at applying various metal/ligand ratios. Fisetin and Cu(II)-fisetin complexes were characterized by potentiometric titrations, UV–Vis (Ultraviolet–visible spectroscopy), EPR, ESI-MS, FTIR and cyclic voltammetry. Their effects on DNA were investigated by using circular dichroism, spectrofluorimetry and gel electrophoresis methods. The copper complex with the ratio of Cu(II)/fisetin 1/2 exhibited significant DNA cleavage activity, followed by complete degradation of DNA. The influence of copper(II) ions on antioxidant activity of fisetin in vitro has been studied using DPPH, ABTS and mitochondrial assays. The results have pointed out that fisetin or copper complexes can behave both as antioxidants or pro-oxidants. Antimicrobial activity of the compounds has been investigated towards several bacteria and fungi. The copper complex of Cu(II)/fisetin 1/2 ratio showed higher antagonistic activity against bacteria comparing to the ligand and it revealed a promising antifungal activity.

Item Type:Article
ID Code:11923
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fisetin, copper complexes, antioxidant activity, mitochondria, antimicrobial activity
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:Elsevier Science
ISSN:0162-0134
Deposited On:07 Feb 2018 10:10

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