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A Recovery time after warming restores mitochondrial function and improves developmental competence of vitrified ovine oocytes

Succu, Sara and Gadau, Sergio Domenico and Serra, Elisa and Zinellu, Angelo and Carru, Ciriaco and Porcu, Cristian and Naitana, Salvatore and Berlinguer, Fiammetta and Leoni, Giovanni Giuseppe (2018) A Recovery time after warming restores mitochondrial function and improves developmental competence of vitrified ovine oocytes. Theriogenology, Vol. 110 , p. 18-26. ISSN 0093-691X. Article.

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DOI: 10.1016/j.theriogenology.2017.12.031

Abstract

The aim of the present study was to assess the ability of vitrified/warmed oocyte to recover from vitrification-induced damages after warming. In vitro matured, vitrified/warmed ovine oocytes were assessed for developmental competence, mitochondrial activity and distribution, ATP, ROS and catalase levels during 6 h of in vitro culture using fresh oocytes as control. ATP content in vitrified oocytes was lower than control during 4 h of post warming culture (p < .01). Vitrified oocytes were able to fill this gap only after 6 h of post-warming incubation. Moreover, mitochondrial activity was significantly lower (p < 0.01) in vitrified oocytes compared to controls, and this difference was maintained up to 2 h of incubation. Then the activity increased and at 4 h it was higher compared to controls (p < 0.01). These oocytes showed an increasing rate of clustered distribution of mitochondria which was lower than controls during the first 4 h of post warming culture (p < 0.01). ROS level was significantly higher at 0 h in vitrified compared to control oocytes and this difference was maintained also at 2 h and 6 h of incubation (p < 0.01). Catalase level was higher in vitrified oocytes than controls (p < 0.01) during the entire culture period. Cleavage and blastocyst rates were lower in vitrified oocytes compared to control ones during the two first time point of incubation period (p < .01), indeed they increased significantly from 0 to 4 h of incubation post warming (p < 0.01). The study demonstrated that vitrified/warmed oocytes need an extra time to restore damage due to cryopreservation procedures and to increase their developmental potential. Thus, time of damage recovery after vitrification could be used to standardize the vitrification protocols and to improve the developmental competence of vitrified/warmed oocytes.

Item Type:Article
ID Code:11916
Status:Published
Refereed:Yes
Uncontrolled Keywords:Oocyte, vitrification, restoration, energetic status, blastocyst yield
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0093-691X
Deposited On:06 Feb 2018 12:46

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