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Effects of crp deletion in Salmonella enterica serotype Gallinarum

Rosu, Valentina and Chadfield, Mark S. and Santona, Antonella and Christensen, Jens P. and Thomsen, Line E. and Rubino, Salvatore and Olsen, John Elmerdahl (2007) Effects of crp deletion in Salmonella enterica serotype Gallinarum. Acta Veterinaria Scandinavica, Vol. 49 (14), p. 1-7. ISSN 1751-0147. Article.

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DOI: 10.1186/1751-0147-49-14

Abstract

Background. Salmonella enterica serotype Gallinarum (S. Gallinarum) remains an important pathogen of poultry, especially in developing countries. There is a need to develop effective and safe vaccines. In the current study, the effect of crp deletion was investigated with respect to virulence and biochemical properties and the possible use of a deletion mutant as vaccine candidate was preliminarily tested. Methods. Mutants were constructed in S. Gallinarum by P22 transduction from Salmonella Typhimurium (S. Typhimurium) with deletion of the crp gene. The effect was characterized by measuring biochemical properties and by testing of invasion in a chicken loop model and by challenge of six-day-old chickens. Further, birds were immunized with the deleted strain and challenged with the wild type isolate. Results. The crp deletions caused complete attenuation of S. Gallinarum. This was shown by ileal loop experiments not to be due to significantly reduced invasion. Strains with such deletions may have vaccine potential, since oral inoculatoin with S. Gallinarum Δcrp completely protected against challenge with the same dose of wild type S. Gallinarum ten days post immunization. Interestingly, the mutations did not cause the same biochemical and growth changes to the two biotypes of S. Gallinarum. All biochemical effects but not virulence could be complemented by providing an intact crp-gene from S. Typhimurium on the plasmid pSD110. Conclusion. Transduction of a Tn10 disrupted crp gene from S. Typhimurium caused attenuation in S. Gallinarum and mutated strains are possible candidates for live vaccines against fowl typhoid.

Item Type:Article
ID Code:119
Status:Published
Refereed:Yes
Uncontrolled Keywords:S. Gallinarum, chicken infection, PCR analyses, phenotypic characterization
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:BioMed Central
ISSN:1751-0147
Copyright Holders:© 2007 Rosu et al; licensee BioMed Central
Deposited On:18 Aug 2009 10:01

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