Ragaglia, Giovanni (2018) Studi sulla diversità della comunità batterica associata alla simbiosi ectomicorrizica tra Tuber borchii e Quercus ilex in differenti ecosistemi forestali della Sardegna. Doctoral Thesis.
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The bacterial component of the soil and of the ascocarps of the Tuber borchii sampled in two holm oaks grown on soils with different rock matrix was detected, in order to evaluate any quantitative-qualitative differences between the sampling sites.
The chemical analysis carried out on soils samples have highlighted their main physics and chemical characteristics.
The microbial communities extracted from the different soil samples were assessed both taxonomically, by sequencing the rRNA of the ribosomal 16S region of 184 bacterial isolates, and on a functional basis with the use of Ecoplates Biolog. The microbial communities related to the ascocarp micro-habitat were evaluated with GEN III Biolog system (36 isolates).
The study has shown how the two microbial communities of the soil of the two sites, even if they are different by taxonomic composition, are identical from a metabolic-functional point of view.
In addition, differences in the composition of bacterial communities are observed: among the fruiting bodies sampled by the two sites, between ascocarp and ascocarpic soil and between ascocarp and forest soil, thus corroborating the hypothesis that every micro-environment detected in this study is an ecological niche that selects specific bacterial communities.
PGPR, saprophytes, fungi and human pathogens were detected among the bacteria detected in the various ascocarps.
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