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Human serum albumin increases the stability of green tea catechins in aqueous physiological conditions

Zinellu, Angelo and Sotgia, Salvatore and Scanu, Bastianina and Forteschi, Mauro and Giordo, Roberta and Cossu, Annalisa and Posadino, Anna Maria and Carru, Ciriaco and Pintus, Gianfranco (2015) Human serum albumin increases the stability of green tea catechins in aqueous physiological conditions. PLoS One, Vol. 10 (7), e0134690. ISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0134690


Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research.

Item Type:Article
ID Code:11765
Uncontrolled Keywords:Electrocardiography, cysteine, serum albumin, albumins, capillary electrophoresis, tea, antioxidants, blood plasma
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Public Library of Science
Copyright Holders:© 2015 Zinellu et al.
Deposited On:14 Jun 2017 16:11

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