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The First mitogenome of the Cyprus mouflon (Ovis gmelini ophion): new insights into the phylogeny of the genus Ovis

Sanna, Daria and Barbato, Mario and Hadjisterkotis, Eleftherios and Cossu, Piero and Decandia, Luca and Trova, Sandro and Pirastru, Monica and Leoni, Giovanni Giuseppe and Naitana, Salvatore and Francalacci, Paolo and Masala, Bruno Lucio and Manca, Laura and Mereu, Paolo (2015) The First mitogenome of the Cyprus mouflon (Ovis gmelini ophion): new insights into the phylogeny of the genus Ovis. PLoS One, Vol. 10 (12), e0144257. ISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0144257

Abstract

Sheep are thought to have been one of the first livestock to be domesticated in the Near East, thus playing an important role in human history. The current whole mitochondrial genome phylogeny for the genus Ovis is based on: the five main domestic haplogroups occurring among sheep (O. aries), along with molecular data from two wild European mouflons, three urials, and one argali. With the aim to shed some further light on the phylogenetic relationship within this genus, the first complete mitochondrial genome sequence of a Cypriot mouflon (O. gmelini ophion) is here reported. Phylogenetic analyses were performed using a dataset of whole Ovis mitogenomes as well as D-loop sequences. The concatenated sequence of 28 mitochondrial genes of one Cypriot mouflon, and the D-loop sequence of three Cypriot mouflons were compared to sequences obtained from samples representatives of the five domestic sheep haplogroups along with samples of the extant wild and feral sheep. The sample included also individuals from the Mediterranean islands of Sardinia and Corsica hosting remnants of the first wave of domestication that likely went then back to feral life. The divergence time between branches in the phylogenetic tree has been calculated using seven different calibration points by means of Bayesian and Maximum Likelihood inferences. Results suggest that urial (O. vignei) and argali (O. ammon) diverged from domestic sheep about 0.89 and 1.11 million years ago (MYA), respectively; and dates the earliest radiation of domestic sheep common ancestor at around 0.3 MYA. Additionally, our data suggest that the rise of the modern sheep haplogroups happened in the span of time between six and 32 thousand years ago (KYA). A close phylogenetic relationship between the Cypriot and the Anatolian mouflon carrying the X haplotype was detected. The genetic distance between this group and the other ovine haplogroups supports the hypothesis that it may be a new haplogroup never described before. Furthermore, the updated phylogenetic tree presented in this study determines a finer classification of ovine species and may help to classify more accurately new mitogenomes within the established haplogroups so far identified.

Item Type:Article
ID Code:11721
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sheep, domestic animals, haplogroups, phylogenetic analysis, haplotypes, mitochondrial DNA, sequence analysis, livestock
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/18 Genetica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze della Natura e del Territorio
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Public Library of Science
ISSN:1932-6203
Copyright Holders:© 2015 Sanna et al.
Deposited On:26 May 2017 11:28

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