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Arginines plasma concentration and oxidative stress in mild to moderate COPD

Zinellu, Angelo and Fois, Alessandro Giuseppe and Sotgia, Salvatore and Sotgiu, Elisabetta and Zinellu, Elisabetta and Bifulco, Fabiana and Mangoni, Arduino A. and Pirina, Pietro and Carru, Ciriaco (2016) Arginines plasma concentration and oxidative stress in mild to moderate COPD. PLoS One, Vol. 11 (8), e0160237. ISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0160237

Abstract

Background: Elevated plasma concentrations of the endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) have been observed in respiratory conditions such as asthma and cystic fibrosis. Since oxidative stress has been shown to increase the activity of arginine methylating enzymes, hence increased ADMA synthesis, and to reduce ADMA degrading enzymes, hence increased ADMA concentrations, we assessed methylated arginines concentrations in chronic obstructive pulmonary disease (COPD), a disease characterized by increased oxidative stress.
Methods: Plasma arginine, ADMA and symmetric dimethylarginine (SDMA), oxidative stress markers (thiobarbituric acid reactive substances, TBARS, and plasma proteins SH, PSH) and antioxidants (taurine and paraoxonase 1, PON1, activity) were measured in 43 COPD patients with mild (n = 29) or moderate (n = 14) disease and 43 age- and sex-matched controls.
Results: TBARS significantly increased with COPD presence and severity (median 2.93 vs 3.18 vs 3.64 μmol/L, respectively in controls, mild and moderate group, p<0.0001 by ANOVA) whereas PSH decreased (6.69±1.15 vs 6.04±0.85 vs 5.33±0.96 μmol/gr prot, p<0.0001 by ANOVA). Increased ADMA/arginine ratio, primarily due to reduced arginine concentrations, was also observed with COPD presence and severity (median 0.0067 vs 0.0075 vs 0.0100, p<0.0001 by ANOVA). In multiple logistic regression analysis, only TBARS (OR 0.44, 95% CI 0.25–0.77; p = 0.0045) and ADMA/Arginine ratio (OR 1.72, 95% CI 2.27–13.05; p = 0.02) were independently associated with COPD severity.
Conclusion: COPD presence and severity are associated with increased oxidative stress and alterations in arginine metabolism. The reduced arginine concentrations in COPD may offer a new target for therapeutic interventions increasing arginine availability.

Item Type:Article
ID Code:11710
Status:Published
Refereed:Yes
Uncontrolled Keywords:Chronic obstructive pulmonary disease, arginine, regression analysis, oxidative stress, methylation, nitric oxide, neutrophils, antioxidants
Subjects:Area 06 - Scienze mediche > MED/10 Malattie dell'apparato respiratorio
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > AOU -Azienda Ospedaliero Universitaria, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Public Library of Science
ISSN:1932-6203
Copyright Holders:© 2016 Zinellu et al.
Deposited On:17 May 2017 16:09

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