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Ancestral European roots of Helicobacter pylori in India

Devi, Sundru Manjulata and Ahmed, Irshad and Francalacci, Paolo and Hussain, M. Abid and Akhter, Yusuf and Alvi, Ayesha and Sechi, Leonardo Antonio and Mégraud, Francis and Ahmed, Niyaz (2007) Ancestral European roots of Helicobacter pylori in India. BMC Genomics, Vol. 8 (184), p. 1-11. ISSN 1471-2164. Article.

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DOI: 10.1186/1471-2164-8-184

Abstract

Background. The human gastric pathogen Helicobacter pylori is co-evolved with its host and therefore, origins and expansion of multiple populations and sub populations of H. pylori mirror ancient human migrations. Ancestral origins of H. pylori in the vast Indian subcontinent are debatable. It is not clear how different waves of human migrations in South Asia shaped the population structure of H. pylori. We tried to address these issues through mapping genetic origins of present day H. pylori in India and their genomic comparison with hundreds of isolates from different geographic regions.
Results. We attempted to dissect genetic identity of strains by multilocus sequence typing (MLST) of the 7 housekeeping genes (atpA, efp, ureI, ppa, mutY, trpC, yphC) and phylogeographic analysis of haplotypes using MEGA and NETWORK software while incorporating DNA sequences and genotyping data of whole cag pathogenicity-islands (cagPAI). The distribution of cagPAI genes within these strains was analyzed by using PCR and the geographic type of cagA phosphorylation motif EPIYA was determined by gene sequencing. All the isolates analyzed revealed European ancestry and belonged to H. pylori sub-population, hpEurope. The cagPAI harbored by Indian strains revealed European features upon PCR based analysis and whole PAI sequencing.
Conclusion. These observations suggest that H. pylori strains in India share ancestral origins with their European counterparts. Further, non-existence of other sub-populations such as hpAfrica and hpEastAsia, at least in our collection of isolates, suggest that the hpEurope strains enjoyed a special fitness advantage in Indian stomachs to out-compete any endogenous strains. These results also might support hypotheses related to gene flow in India through Indo-Aryans and arrival of Neolithic practices and languages from the Fertile Crescent.

Item Type:Article
ID Code:116
Status:Published
Refereed:Yes
Uncontrolled Keywords:DNA isolates, diagnostic PCR, epidemiological genotyping, cagPAI sequence, cagA sequence
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/18 Genetica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > ISOGEM-International Society for Genomic and Evolutionary Microbiology, Sassari
001 Università di Sassari > 01 Dipartimenti > Zoologia e genetica evoluzionistica
Publisher:BioMed Central
ISSN:1471-2164
Deposited On:18 Aug 2009 10:01

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