Russo, Andrea and Ragusa, Marco and Barbagallo, Cristina and Longo, Antonio and Avitabile, Teresio and Uva, Maurizio Giacinto and Bonfiglio, Vincenza and Toro, Mario Damiano and Caltabiano, Rosario and Mariotti, Cesare and Boscia, Francesco and Romano, Mario R. and Di Pietro, Cinzia Santa and Barbagallo, Davide and Purrello, Michele and Reibaldi, Michele (2017) MiRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole. PLoS One, Vol. 12 (3), e0174297. ISSN 1932-6203. Article.
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The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group.
In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors.
Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p: < 0.00004), mir-24 (FC -7.52, p: < 0.004) and miR-142-3p (FC -5.32, p: < 0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM.
The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.
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