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MiRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

Russo, Andrea and Ragusa, Marco and Barbagallo, Cristina and Longo, Antonio and Avitabile, Teresio and Uva, Maurizio Giacinto and Bonfiglio, Vincenza and Toro, Mario Damiano and Caltabiano, Rosario and Mariotti, Cesare and Boscia, Francesco and Romano, Mario R. and Di Pietro, Cinzia Santa and Barbagallo, Davide and Purrello, Michele and Reibaldi, Michele (2017) MiRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole. PLoS One, Vol. 12 (3), e0174297. ISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0174297

Abstract

Purpose
The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group.
Methods
In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors.
Results
Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p: < 0.00004), mir-24 (FC -7.52, p: < 0.004) and miR-142-3p (FC -5.32, p: < 0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM.
Conclusion
The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.

Item Type:Article
ID Code:11572
Status:Published
Refereed:Yes
Uncontrolled Keywords:MicroRNAs, eyes, fibrosis, RNA extraction, vitrectomy, exosomes, eye diseases, surgical and invasive medical procedures
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
Publisher:Public Library of Science
ISSN:1932-6203
Copyright Holders:© 2017 Russo et al.
Deposited On:11 Apr 2017 10:23

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