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De novo synthesis of budding yeast DNA polymerase alpha and POL1 transcription at the G1/S boundary are not required for entrance into S phase

Muzi Falconi, Marco and Piseri, Anna and Ferrari, Marina and Lucchini, Giovanna and Plevani, Paolo and Foiani, Marco (1993) De novosynthesis of budding yeast DNA polymerase alpha and POL1 transcription at the G1/S boundary are not required for entrance into S phase. Proceedings of The National Academy of Sciences, Vol. 90 (22), p. 10519-10523. eISSN 1091-6490. Article.

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Abstract

The POL1 gene, encoding DNA polymerase α(pol α) in Saccharomyces cerevisiae, is transiently transcribed during the cell cycle at the G1/S phase boundary. Here we show that yeast pol α is present at every stage of the cell cycle, and its level only slightly increases following the peak of POL1 transcription. POL1 mRNA synthesis driven by a GAL1 promoter can be completely abolished without affecting the growth rate of logarithmically growing yeast cultures for several cell divisions, although the amount of the pol α polypeptide drops below the physiological level. Moreover, α-factor-arrested cells can enter S phase and divide synchronously even if POL1 transcription is abolished. These results indicate that the level of yeast pol α is not rate limiting and de novo synthesis of the enzyme is not required for entrance into S phase.

Item Type:Article
ID Code:1155
Status:Published
Refereed:Yes
Uncontrolled Keywords:Saccharomyces cerevisiae, yeasts, S phase, DNA polymerase, biosynthesis, scomycetes, thallophyta, nucleotidyltransferases
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:The National Academy of Sciences of the USA
eISSN:1091-6490
Publisher Policy:Depositato per gentile concessione dell'Editore. È vietato duplicare e postare il PDF. E' consentito l'uso a solo scopo di studio e di ricerca.
Deposited On:18 Aug 2009 10:04

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