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Production and lambing rate of blastocysts derived from in vitro matured oocytes after gonadotropin treatment of prepubertal ewes

Ledda, Sergio and Bogliolo, Luisa and Leoni, Giovanni Giuseppe and Naitana, Salvatore (1999) Production and lambing rate of blastocysts derived from in vitro matured oocytes after gonadotropin treatment of prepubertal ewes. Journal of Animal Science, Vol. 77 (8), p. 2234-2239. eISSN 1525-3163. Article.

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Abstract

The aim of this study was to evaluate the effect of gonadotropin treatment on the in vitro maturation, blastocyst production, and developmental potential to term of oocytes collected from Sardinian neonatal and prepubertal ewes at 4 to 6 wk of age. Cumulus-oocyte complexes were recovered at 24 h after withdrawal of a 1/6th size progestagenated pessary from the donors, of which each received 120 IU FSH/LH and 400 IU PMSG in a single dose 36 h before sponge removal. Treated donors produced a greater ( P < .01) number of oocytes per animal (86.2 ± 7.9) compared with slaughterhouse (untreated) prepubertal ewes (55.5 ± 6.1) of the same age or with treated neonatal ewes (6.1 ± 0.7) 10 d old. During oocyte maturation, there were no differences in the percentage of germinal vesicle break-down (78.08 vs 74.24), metaphase I (89.13 vs 87.18), and metaphase II (77.91 vs 76.38) when evaluated after 8, 14, and 24 h of maturation, respectively, between oocytes from treated and slaughterhouse (untreated) prepubertal ewes. The embryo cleavage (71.1 vs 73.7) and blastocyst rates (22.2 vs 19.8) were similar in the treated and the untreated prepubertal ewes after transfer of in vitro matured oocytes into ligated oviducts of temporary recipients. The in vitro viability rates of vitrified blastocysts (81.2 vs 76.9) and the in vivo survival rates (46.1 vs 50.0) of embryos derived from in vitro matured and in vivo fertilized oocytes showed no difference. The data suggest that gonadotropin treatment increases oocyte production per animal but has no effect on oocyte quality because embryo production and lambing rates of blastocysts derived from in vitro matured oocytes were not markedly different from those derived from untreated prepubertal ewes of the same age.

Item Type:Article
ID Code:1150
Status:Published
Refereed:Yes
Uncontrolled Keywords:Gonadotropins, oocytes, blastocyst, lambing, sheep, Sardinia
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:American Society of Animal Science
eISSN:1525-3163
Deposited On:18 Aug 2009 10:04

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