De Muro, Pierina and Capobianco, Giampiero and Lepedda, Antonio Junior and Nieddu, Gabriele and Formato, Marilena and Tram, Nguyen Hai Quy and Idini, Michela and Dessole, Francesco and Dessole, Salvatore (2016) Plasma PP13 and urinary GAGs/PGs as early markers of pre-eclampsia. Archives of Gynecology and Obstetrics, Vol. 294 (5), p. 959-965. ISSN 0932-0067. Article.
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To evaluate at 11–13 weeks’ gestation biochemical markers that may predict complications of pregnancy such as pre-eclampsia, proteinuria, and hypertension.
Analyses were performed on first-morning urine and plasma samples from first trimester pregnant women with increased risk of developing pre-eclampsia such as positive personal or family history of cardiovascular disease and diabetes mellitus. A total of 62 women were enrolled, 24 of them presented complications such as pre-eclampsia, proteinuria, and hypertension during pregnancy. The remaining 38 women had a physiological course of pregnancy and formed the reference group. Urine glycosaminoglycans/proteoglycans (GAGs/PGs) distribution was determined by electrophoresis on cellulose acetate strips. Urinary N-acetyl-β-glucosaminidase was estimated kinetically. Plasma levels of placental protein 13 (PP13) were measured by enzyme-linked immunosorbent assay.
No significant differences in total GAG excretion and N-acetyl-β-glucosaminidase (NAG) concentration were observed between the two groups of pregnant women, whereas we detected increased relative content of total urinary trypsin inhibitor (UTI plus low-sulfated chondroitin sulfate) (p = 0.001) and reduced excretion of heparan sulfate (p = 0.007) and chondroitin sulfate (p = 0.011) in women presenting with pregnancy complications respect to controls. Plasma levels of PP13 were significantly reduced in the group of women who went on to develop complications compared with controls (p = 0.022).
The reduced plasma levels of PP13 and the alteration of the relative content of urinary GAGs and PGs observed in our study could be a promising tool for the prediction of pre-eclampsia in an early stage of pregnancy.
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