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CRE recombinase-inducible RNA interference mediated by lentiviral vectors

Tiscornia, Gustavo and Tergaonkar, Vinay and Galimi, Francesco and Verma, Inder M. (2004) CRE recombinase-inducible RNA interference mediated by lentiviral vectors. Proceedings of The National Academy of Sciences, Vol. 101 (19), p. 7347-7351. eISSN 1091-6490. Article.

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DOI: 10.1073/pnas.0402107101

Abstract

Recently, several systems designed to trigger RNA interference by using small hairpin RNA driven by polymerase III promoters have been described. Here, we report a lentiviral-mediated small interfering RNA delivery system that can be induced by CRE recombinase. The system consists of a lentiviral vector carrying a mouse U6 promoter that is separated from a small hairpin RNA by a random DNA stuffer sequence flanked by modified loxP sites. The silencing cassette is not expressed until activated by addition of CRE recombinase delivered by a lentiviral vector. We have used this system to show specific down-regulation of GFP and two endogenous genes (the tumor suppressor p53 and the NF-κB transcription factor subunit p65) in vitro. Furthermore, down-regulation of both p53 and p65 resulted in the expected effect on downstream genes and cellular phenotype. We foresee multiple applications of this system both in vitro and in vivo to down-regulate specific targets in a tissue-specific and localized manner.

Item Type:Article
ID Code:1144
Status:Published
Refereed:Yes
Uncontrolled Keywords:CRE, RNA, lentiviral vectors, genetics
Subjects:Area 05 - Scienze biologiche > BIO/17 Istologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:The National Academy of Sciences of the USA
eISSN:1091-6490
Publisher Policy:Depositato per gentile concessione dell'Editore. È vietato duplicare e postare il PDF. E' consentito l'uso a solo scopo di studio e di ricerca.
Deposited On:18 Aug 2009 10:04

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