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Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto's thyroiditis patients

Masala, Speranza and Cossu, Davide and Niegowska, Magdalena and Mameli, Giuseppe and Paccagnini, Daniela and Sechi, Leonardo Antonio (2015) Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto's thyroiditis patients. The Journal of Infection in Developing Countries, Vol. 9 (6), p. 631-634. ISSN 1972-2680. Article.

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DOI: 10.3855/jidc.6284

Abstract

Introduction: The Helicobacter pylori (HP) reinfection rate seems to be higher in developing countries than in developed ones. An increased seroprevalence of HP has also been reported in patients with type 1 diabetes (T1D) and Hashimoto’s thyroiditis (HT). Mycobacterium avium subsp. paratuberculosis (MAP) has been linked to both T1D and HT. Quite a few lines of evidence indicate that autoantibodies against several epitopes belonging to human zinc transporter 8 (ZnT8) cross-recognize the homologous MAP3865c epitopes in both T1D and HT patients. HP may play a role in HT disease, most likely acting through a molecular mimicry mechanism that targets ZnT8 as reported for MAP and the two autoimmune diseases.
Methodology: An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies against several epitopes deriving from HP proteins, which are highly homologous to the immunodominant ZnT8 peptides previously identified: ZnT8178–186 and ZnT8186–194.
Results: None of the HP peptides tested were significantly recognized when the humoral responses of 92 HT patients and 91 healthy volunteers were analyzed.
Conclusions: These findings do not support a triggering role for HP (through ZnT8 mimicking) in HT. If a molecular mimicry phenomenon is taking place, it involves a different self-antigen. Moreover, the negative outcome of the experiments performed stresses the fact that sharing stretches of sequence homology is relevant, but not enough to trigger an antibody-mediated cross-recognition.

Item Type:Article
ID Code:11331
Status:Published
Refereed:Yes
Uncontrolled Keywords:Zinc transporter 8, Hashimoto’s thyroiditis, Helicobacter pylori
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/19 Microbiologia generale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:OLoeP
ISSN:1972-2680
Copyright Holders:© 2015 Masala et al.
Deposited On:10 Apr 2017 10:31

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