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Gas chromatography-mass spectrometry metabolomics of goat milk with different polymorphism at the αS1-casein genotype locus

Caboni, Pierluigi and Murgia, Antonio and Porcu, Alessandra and Demuru, Martina and Pulina, Giuseppe and Nudda, Anna (2016) Gas chromatography-mass spectrometry metabolomics of goat milk with different polymorphism at the αS1-casein genotype locus. Journal of Dairy Science, Vol. 99 (8), p. 6046-6051. ISSN 0022-0302. Article.

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DOI: 10.3168/jds.2015-10537

Abstract

Hyphenated gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis techniques were used to uncover milk metabolite differences in different αS1-casein genotypes of goats. By a discriminant GC-MS metabolomics approach, we characterized milk polar metabolites of 28 goats. Animals were selected on the basis of their genotypes as 7 goats classified heterozygous for weak or null alleles, 5 for the genotype EE, 9 for the genotypes AE and BE, and finally 7 for the strong genotype AA. Low molecular weight polar metabolite profile was tightly related to the different goat genotypes, milk production, and protein levels. Results of multivariate statistical analysis of GC-MS data demonstrate that different heterozygous and homozygous genotypes expressed different metabolites such as citric and aconitic acid for the strong allele class with different sugars and polyols for the weak class.

Item Type:Article
ID Code:11310
Status:Published
Refereed:Yes
Uncontrolled Keywords:Multivariate analysis, genotypes, aconitic acid, levoglucosan
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/15 Scienze e tecnologie alimentari
Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Publisher:American Dairy Science Association
ISSN:0022-0302
Deposited On:10 Apr 2017 16:35

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