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Identification of Chattonella (Raphidophyceae) species in long-term phytoplankton samples from Santa Giusta Lagoon, Italy.

Stacca, Daniela and Satta, Cecilia Teodora and Casabianca, Silvia and Penna, Antonella and Padedda, Bachisio Mario and Sechi, Nicola and Lugliè, Antonella Gesuina Laura (2016) Identification of Chattonella (Raphidophyceae) species in long-term phytoplankton samples from Santa Giusta Lagoon, Italy. Scientia Marina, Vol. 80 (1), p. 17-25. ISSN 0214-8358. eISSN 1886-8134. Article.

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DOI: 10.3989/scimar.04292.09A

Abstract

Chattonella species in a Mediterranean lagoon (Santa Giusta Lagoon, Sardinia, Italy) were identified by applying a molecular approach to fixed natural phytoplankton samples collected over the last two decades. Like the other raphidophytes, Chattonella cells are naked and lose their shape when fixed, making species identification difficult on the basis of their morphological characteristics. Employing species-specific primers (oBTG-005-F, oBTG-027-R, oBTG-028-R) for the amplification of the ITS-5.8S rDNA region, we established the occurrence of C. subsalsa in fixed natural phytoplankton samples collected in coincidence with fish death events. Additionally, we established the presence of the recently discovered C. cf. subsalsa Adriatic genotype by analysing cellular cultures obtained from the same lagoon in 2013. This is the second worldwide record of C. cf. subsalsa Adriatic genotype. Our results revealed that the species-specific primers oBTG-005-F and oBTG-028-R distinguished this new genotype only when present singularly. This study provides valuable data that increase knowledge of C. subsalsa genotypes and of the long-term occurrence of Chattonella blooms in a transitional ecosystem through the use of samples up to 20 years old.

Item Type:Article
ID Code:11277
Status:Published
Refereed:Yes
Uncontrolled Keywords:Chattonella subsalsa genotypes, transitional ecosystems, harmful algal blooms, LTER-Italy, ITS-5.8S rDNA, LSU rDNA
Subjects:Area 05 - Scienze biologiche > BIO/07 Ecologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Architettura, Design e Urbanistica
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze della Natura e del Territorio
Publisher:Institut de Ciències del Mar de Barcelona (CSIC)
ISSN:0214-8358
eISSN:1886-8134
Copyright Holders:© 2016 CSIC
Deposited On:26 Apr 2016 11:51

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