Spano , Giovanna Maria (2016) Monitoraggio real-time della neurochimica corticale in topi liberi di muoversi durante il sonno, la privazione di sonno e la veglia medinte microdialisi in vivo e neurosensori amperometrici. Doctoral Thesis.
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Aim: for millennia, mankind has tried to understand the function and the importance of sleep and this is a mystery that biology has yet to solve. The purpose of this research was study neurochemistry during sleep, sleep deprivation and wakefulness by means of in-vivo microdialysis and amperometric neurosensors.
Method: the neurochemistry of the brain was studied by the combination of electrochemical and microdialysis techniques, electroencephalographic (EEG) and electromyographic (EMG) recordings. The mice were implanted with three electrodes for electroencephalographic recordings and two microdialysis probes. During the experiment, samples were taken every 15 minutes and then analyzed by UHPLC/CD ORBITRAP that allows to analyze all the molecules present the sample. Moreover, the biochemical dynamics of Ascorbic Acid (AA) have been extensively studied both in vitro and in vivo by the development of a nanostructured amperometric microsensor decorated with multiwall carbonanotubes (MWCNTs).
Results: through multiple experiments it was possible to study the variation in levels of cortical AA in freely moving mice during sleep and sleep deprivation, furthermore, the microdialysis technique, combined with EEG and EMG, allowed to monitor extracellular molecules (as neurotransmitters) that are involved in the neurochemistry of sleep, sleep deprivation and wakefulness.
Conclusion: understand the biology that is the basis of sleep is the goal of many researchers. It seems to be involved in different processes such as learning and memory, also, his disorders are the basis of diseases (i.e. depression). This study allows, for the first time, to have an overview of the neurochemistry of sleep contribute, therefore, to better understanding of its mechanisms and functions and laying the groundwork for future studies.
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