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Molecular and antigenetic characterization of Streptococcus suis serotype 2 isolates in Central Vietnam

Nguyen , Hoang Bach (2016) Molecular and antigenetic characterization of Streptococcus suis serotype 2 isolates in Central Vietnam. Doctoral Thesis.

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Streptococcus suis is a Gram-positive bacterium with clinical relevance in pigs and is emerging in human. There are 35 S. suis serotypes have been distinguished to date, of which serotype 2 is most commonly associated with disease in human and pigs, worldwide. Recently, S. suis emerged as a zoonotic agent in human in contact with infected pigs or with their products. More recently, several studies from Southeast Asia demonstrated that S. suis can cause adult endocarditis, septicemia, arthritis and especially meningitis with high fatality rate and severe neurological sequelae. Multilocus enzyme electrophoresis, restriction endonuclease analysis with HaeIII, ribotyping, and pulsed-field gel electrophoresis have been used to determine S. suis strains epidemiological relationships. Characterization of the 16S-23S ribosomal (r) DNA intergenic spacer region (ITS) has also been used to compare bacterial strains and to identify species within the genus Streptococcus, including S. suis.
In this study, a specific and sensitive PCR assay for fast detection of S. suis serotype 2 is crucial to meningitis treatment based on the S. suis serotype 2 cps2J gene was developed and evaluated for diagnosis in meningitis patients hospitalized in central Viet Nam. The intergenic spacer region was amplified by traditional PCR from strains obtained in this study and sequenced. ITS - based phylogenetic analysis is also presented and discussed. We also identified and characterized the main immunodominant antigens of Streptococcus suis serotype 2 based on the immunoproteomics approach.

Item Type:Doctoral Thesis
ID Code:11115
Contributors:Alberti, Alberto and Le, Van An
Publisher:Universita' degli studi di Sassari
Uncontrolled Keywords:Streptococcus suis serotype 2, meningitis, real-time PCR, intergenic spacer (ITS), immunoproteomics, antigenic protein
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/05 Malattie infettive degli animali domestici
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Cicli, scuole e corsi:Ciclo 28 > Scienze biomolecolari e biotecnologiche > Microbiologia e immunologia
Deposited On:03 May 2016 08:45

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