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Use of molecular identification techniques for the study of parasitoids of the chestnut gall wasp

Schiaffino, Angela (2016) Use of molecular identification techniques for the study of parasitoids of the chestnut gall wasp. Doctoral Thesis.

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The chestnut gall wasp Dryocosmus kuriphilus Yasumatsu (Hymenoptera, Cynipidae) is considered as a major pest of Castanea species worldwide. A three-year monitoring of the indigenous parasitoids of this pest was performed by collecting specimens inside the galls. Each specimen was processed by molecular analysis. DNA was analysed by amplification and sequencing of the COI gene, coding for cytochrome c oxydase subunit 1. Each sequence was compared with reference sequences from adults sampled in Sardinia and those present both in GenBank and Barcode of Life Database (BOLD). This procedure enabled us to identify all immature life stages, included larvae and pupae that cannot be identified otherwise. The identified species belong to 7 genera of the superfamily Chalcidoidea: Eupelmus (Eupelmidae), Eurytoma and Sycophila (Eurytomidae), Ormyrus (Ormyridae), Mesopolobus (Pteromalidae), Megastigmus and Torymus (Torymidae). Among the identified taxa we found a few unknown species and also some sibling ones. These findings will make it possible to elucidate the relationships between the indigenous parasitoids and the host pest and to verify the spreading of the introduced exotic antagonist Torymus sinensis in order to plan new control strategies.

Item Type:Doctoral Thesis
ID Code:11090
Contributors:Pantaleoni, Roberto Antonio
Publisher:Universita' degli studi di Sassari
Uncontrolled Keywords:DNA barcoding, chalcidoid wasps, Dryocosmus kuriphilus, Castanea, Sardinia
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/11 Entomologia generale e applicata
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Cicli, scuole e corsi:Ciclo 28 > Scienze e biotecnologie dei sistemi agrari e forestali e delle produzioni alimentari > Monitoraggio e controllo degli ecosistemi forestali in ambiente mediterraneo
Deposited On:03 May 2016 10:03

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