Totiger, Smitha Tulasigeri (2016) The Chondropaties: study of antagonism between growth factors and reactive oxygen species; clinical applications in the early stage of chondral lesions. Doctoral Thesis.
Oxidative stress is one of the risk factors for chondropathies. Deterioration of the cell functions due to oxidative stress has been found to be the initial stage changes involving disruption of the cartilage homeostasis. Eventually these changes progress to the final stage of osteoarthritis involving series of catabolic damage mediated by pro-inflammatory cytokines, MMPs, aggrecanases and cell death. Our study investigated the role of fluid, isolated from post operation knee drainage (emartri), of patients who underwent prosthesis replacement surgery, against hydrogen peroxide (H2O2) - induced oxidative stress on normal human articular chondrocytes (HCa). HCa were pre-treated with emartri and then exposed to H2O2 to mimic oxidative stress. Intracellular reactive oxygen species (ROS) levels were measured by using the molecular probe H2DCFDA; intracellular oxidative status in both the cytoplasm and mitochondrial compartment was measured by using HCa infected with lentiviral particle harbouring the redox sensing green fluorescent protein (roGFP); cell proliferation was determined by measuring the rate of DNA synthesis with BrdU incorporation. Moreover, superoxide dismutase (SOD), catalase and glutathione levels from the cell lysates were measured. Haematic sera from the same patient, obtained post-operation, were included in our study to compare and evaluate the response given by the two types of sera.
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