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Biosensori elettrochimici di tipo amperometrico di potenziale interesse nella caratterizzazione di produzioni agroalimentari

Farre, Roberta (2016) Biosensori elettrochimici di tipo amperometrico di potenziale interesse nella caratterizzazione di produzioni agroalimentari. Doctoral Thesis.

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Abstract

In this study, some thiophene-based conducting polymers, deposited on a WE surface, are used as a matrix for the entrapment of enzymes as glucose oxidase (GOx) for glucose detection and tyrosinase (Tyr) for cathecol detection. GOx was covalently immobilized on polythiophene film by cross-linking with 1-Cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate (CMC) (1) as condensing agent or albumin sierum bovine and glutaraldehyde (GA). Tyr was immobilized on the polymeric film by cross-linking with GA, or covalently by activation of carboxylic groups that are present in the some polythiophene structure using a solution of EDC/NHS (0.4/0.1 M). Amperometric response of biosensors at varying the concentration of analyte was recorded in a three-electrode cell containing phosphate buffer (pH 7). For GOx biosensor, a redox mediator (p-benzoquinone), was for electronic-excange. Biosensors performance have been checked in standard samples and for GOx biosensors in real samples.
Another bi-enzymatic biosensor based on Aldehyde dehydrogenase (β-NAD+ dependent) and Diaphorase for 5-hydroxymethyl-2-furaldheyde (HMF) detection was studied (2). Polypyrrole was used as matrix for the enzymes immobilization by entrapment or cross-linking with BSA and GA. Amperometric response of biosensor at varying the concentration of HMF was recorded in a three-electrode cell containing phosphate buffer pH 7,5 and [Fe(CN)6]3- as redox mediator.
1. Liu C. et al. Europ. Polym. J. 2007, Vol. 43, p. 3264-3276.
2. Lomillo M. A. et al. Electroanal. 2006, Vol. 18, p. 2435-2440.

Item Type:Doctoral Thesis
ID Code:11055
Contributors:Sanna, Gavino
Publisher:Universita' degli studi di Sassari
Uncontrolled Keywords:Biosensors, chemistry, mmf, glucose
Subjects:Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Cicli, scuole e corsi:Ciclo 28 > Scienze e tecnologie chimiche > Scienze chimiche
Deposited On:18 Apr 2016 18:02

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