Farre, Roberta (2016) Biosensori elettrochimici di tipo amperometrico di potenziale interesse nella caratterizzazione di produzioni agroalimentari. Doctoral Thesis.
In this study, some thiophene-based conducting polymers, deposited on a WE surface, are used as a matrix for the entrapment of enzymes as glucose oxidase (GOx) for glucose detection and tyrosinase (Tyr) for cathecol detection. GOx was covalently immobilized on polythiophene film by cross-linking with 1-Cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate (CMC) (1) as condensing agent or albumin sierum bovine and glutaraldehyde (GA). Tyr was immobilized on the polymeric film by cross-linking with GA, or covalently by activation of carboxylic groups that are present in the some polythiophene structure using a solution of EDC/NHS (0.4/0.1 M). Amperometric response of biosensors at varying the concentration of analyte was recorded in a three-electrode cell containing phosphate buffer (pH 7). For GOx biosensor, a redox mediator (p-benzoquinone), was for electronic-excange. Biosensors performance have been checked in standard samples and for GOx biosensors in real samples.
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