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Tungsten-induced carcinogenesis in human bronchial epithelial cells

Laulicht, Freda and Brocato, Jason and Cartularo, Laura and Vaughan, Joshua and Wu, Feng and Kluz, Thomas and Sun, Hong and Oksuz, Betul Akgol and Shen, Steven and Peana, Massimiliano Francesco and Medici, Serenella and Zoroddu, Maria Antonietta and Costa, Max (2015) Tungsten-induced carcinogenesis in human bronchial epithelial cells. Toxicology and Applied Pharmacology, Vol. 288 (1), p. 33-39. ISSN 0041-008X. Article.

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DOI: 10.1016/j.taap.2015.07.003

Abstract

Metals such as arsenic, cadmium, beryllium, and nickel are known human carcinogens; however, other transition metals, such as tungsten (W), remain relatively uninvestigated with regard to their potential carcinogenic activity. Tungsten production for industrial and military applications has almost doubled over the past decade and continues to increase. Here, for the first time, we demonstrate tungsten's ability to induce carcinogenic related endpoints including cell transformation, increased migration, xenograft growth in nude mice, and the activation of multiple cancer-related pathways in transformed clones as determined by RNA sequencing. Human bronchial epithelial cell line (Beas-2B) exposed to tungsten developed carcinogenic properties. In a soft agar assay, tungsten-treated cells formed more colonies than controls and the tungsten-transformed clones formed tumors in nude mice. RNA-sequencing data revealed that the tungsten-transformed clones altered the expression of many cancer-associated genes when compared to control clones. Genes involved in lung cancer, leukemia, and general cancer genes were deregulated by tungsten. Taken together, our data show the carcinogenic potential of tungsten. Further tests are needed, including in vivo and human studies, in order to validate tungsten as a carcinogen to humans.

Item Type:Article
ID Code:11024
Status:Published
Refereed:Yes
Uncontrolled Keywords:Tungsten, cancer, Beas-2B, RNA-Seq, in vitro, nude mice
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/15 Biologia farmaceutica
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:Elsevier
ISSN:0041-008X
Deposited On:08 Sep 2015 09:06

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