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The Sheep growth hormone gene polymorphism and its effects on milk traits

Dettori, Maria Luisa and Pazzola, Michele and Pira, Emanuela and Paschino, Pietro and Vacca, Giuseppe Massimo (2015) The Sheep growth hormone gene polymorphism and its effects on milk traits. Journal of Dairy Research, Vol. 82 (2), p. 169-176. eISSN 1469-7629. Article.

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DOI: 10.1017/S0022029915000047

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Abstract

Growth hormone (GH) is encoded by the GH gene, which may be single copy or duplicate in sheep. The two copies of the sheep GH gene (GH1/GH2-N and GH2-Z) were entirely sequenced in one 106 ewes of Sarda breed, in order to highlight sequence polymorphisms and investigate possible association between genetic variants and milk traits. Milk traits included milk yield, fat, protein, casein and lactose percentage. We evidenced 75 nucleotide changes. Transcription factor binding site prediction revealed two sequences potentially recognised by the pituitary-specific transcription factor POU1FI at the GH1/GH2-N gene, which were lost at the promoter of GH2-Z, which might explain the different tissues of expression of GH1/GH2-N (pituitary) and GH2-Z (placenta). Significant differences in milk traits were observed among genotypes at polymorphic loci only for the GH2-Z gene. Sheep with homozygote genotype ss748770547 CC had higher fat percentage (P < 0·01) than TT. SNP ss748770547 was part of a potential transcription factor binding site for C/EBP alpha (CCAAT/Enhancer Binding Protein), which is involved in the regulation of adipogenesis and adipoblast differentiation. SNP ss748770547, located in the GH2-Z gene 5′ flanking region, may be a causal mutation affecting milk fat content. These findings might contribute to the knowledge of the sheep GH locus and might be useful in selection processes in sheep.

Item Type:Article
ID Code:10984
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sheep milk, Sarda sheep breed, growth hormone gene, sheep GH gene, duplicate GH gene
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
001 Università di Sassari > 02 Centri > Centro di competenza biodiversità animale
Publisher:Cambridge University Press
eISSN:1469-7629
Deposited On:05 Jun 2015 09:10

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