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Neurological morphofunctional differentiation induced by REAC technology in PC12: a neuro protective model for Parkinson's disease

Maioli, Margherita and Rinaldi, Salvatore and Migheli, Rossana and Pigliaru, Gianfranco and Rocchitta, Gaia Giovanna Maria and Santaniello, Sara and Basoli, Valentina and Castagna, Alessandro and Fontani, Vania and Ventura, Carlo and Serra, Pier Andrea (2015) Neurological morphofunctional differentiation induced by REAC technology in PC12: a neuro protective model for Parkinson's disease. Scientific reports, Vol. 5 (10439), p. 8. eISSN 2045-2322. Article.

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DOI: 10.1038/srep10439

Abstract

Research for the use of physical means, in order to induce cell differentiation for new therapeutic strategies, is one of the most interesting challenges in the field of regenerative medicine, and then in the treatment of neurodegenerative diseases, Parkinson’s disease (PD) included. The aim of this work is to verify the effect of the radio electric asymmetric conveyer (REAC) technology on the PC12 rat adrenal pheochromocytoma cell line, as they display metabolic features of PD. PC12 cells were cultured with a REAC regenerative tissue optimization treatment (TO-RGN) for a period ranging between 24 and 192 hours. Gene expression analysis of specific neurogenic genes, as neurogenin-1, beta3-tubulin and Nerve growth factor, together with the immunostaining analysis of the specific neuronal protein beta3-tubulin and tyrosine hydroxylase, shows that the number of cells committed toward the neurogenic phenotype was significantly higher in REAC treated cultures, as compared to control untreated cells. Moreover, MTT and Trypan blue proliferation assays highlighted that cell proliferation was significantly reduced in REAC TO-RGN treated cells. These results open new perspectives in neurodegenerative diseases treatment, particularly in PD. Further studies will be needed to better address the therapeutic potential of the REAC technology.

Item Type:Article
ID Code:10972
Status:Published
Refereed:Yes
Uncontrolled Keywords:REAC technology, PC12, nerve growth factor, Parkinson’s disease (PD)
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Nature Publishing Group
eISSN:2045-2322
Copyright Holders:© Maioli, M. et al.
Deposited On:11 Jun 2015 17:34

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