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Proteome changes during yeast-like and pseudohyphal growth in the biofilm-forming yeast Pichia fermentans

Maserti, Bianca Elena and Podda, Alessandra and Giorgetti, Lucia and Del Carratore, Renata and Chevret, Didier and Migheli, Quirico (2015) Proteome changes during yeast-like and pseudohyphal growth in the biofilm-forming yeast Pichia fermentans. Amino Acids, Vol. 47 (6), p. 1091-1106. ISSN 0939-4451. Article.

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DOI: 10.1007/s00726-015-1933-1

Abstract

The Pichia fermentans strain DISAABA 726 is a biofilm-forming yeast that has been proposed as biocontrol agent to control brown rot on apple. How ever, when inoculated on peach, strain 726 shows yeast-like to pseudohyphal transition coupled to a pathogenic behaviour. To identify the proteins potentially involved in such transition process, a comparative proteome analysis of P. fermentans 726 developed on peach (filamentous growth) vs apple (yeast-like growth) was carried out using two-dimensional gel electrophoresis coupled with mass spectrometry analysis. The proteome comparison was also performed between the two different cell morphologies induced in a liquid medium amended with urea (yeast-like cells) or methionine (filamentous cells) to exclude fruit tissue impact on the transition. Seventy-three protein spots showed significant variations in abundance (±twofold, p < 0.01, confidence intervals 99 %) between pseudohyphal vs yeast-like morphology produced on fruits. Among them, 30 proteins changed their levels when the two morphologies were developed in liquid medium. The identified proteins belong to several pathways and functions, such as glycolysis, amino acid synthesis, chaperones, and signalling transduction. The possible role of a group of proteins belonging to the carbohydrate pathway in the metabolic re-organisation during P. fermentans dimorphic transition is discussed.

Item Type:Article
ID Code:10938
Status:Published
Refereed:Yes
Uncontrolled Keywords:Antagonistic yeast, biological control, dimorphic transition, yeast proteome, candida lambica, two-dimensional electrophoresis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Publisher:Springer Wien
ISSN:0939-4451
Deposited On:26 May 2015 09:43

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