Ciulu, Marco and Floris, Ignazio and Nurchi, Valeria Marina and Panzanelli, Angelo and Pilo, Maria Itria and Spano, Nadia and Sanna, Gavino (2015) A Possible freshness marker for royal jelly: formation of 5-hydroxymethyl-2-furaldehyde as a function of storage temperature and time. Journal of Agricultural and Food Chemistry, Vol. 63 (16), p. 4190-4195. ISSN 0021-8561. Article.
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In this article we present a study of the variability of the concentration of 5-hydroxymethyl-2-furaldehyde (HMF) in natural royal jelly (RJ) as a function of its storage temperature (−18, 4, and 25 °C) and time (up to 9 months after harvesting). For this work HMF is evaluated using an RP-HPLC method we previously assessed. While all RJ samples stored at 4 and −18 °C always showed levels of HMF under the limit of detection (0.13 mg kg–1), samples kept at 25 °C showed an exponential increase in the concentration of HMF as a function of the storage time. This behavior and a number of desirable features of the analytical method used (ease of use in routine laboratories, availability of a complete validation protocol specifically developed for RJ, based on consolidated chemical knowledge) allow us to hypothesize the use of HMF as a possible, reliable freshness marker for RJ.
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