Sotgia, Salvatore and Pisanu, Elisabetta and Cambedda, Debora and Pintus, Gianfranco and Carru, Ciriaco and Zinellu, Angelo (2014) Ultra-performance liquid chromatographic determination of L-ergothioneine in commercially available classes of cow milk. Journal of Food Science, Vol. 79 (9), p. C1683-C1687. ISSN 0022-1147. eISSN 1750-3841. Article.
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A new efficient and sensitive precolumn hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) method was established for the quantitative determination of L-ergothioneine (ERT) in milk. After derivatization of ERT with 7-diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin, chromatographic separation was achieved in a fairly short time, less than 5 min, on a 100 × 2.1 mm Waters Cortecs UPLC HILIC 1.6- µm column, by using a mixture of 30 mmol/L ammonium acetate/acetonitrile (10:90, v/v) as a mobile phase flowing isocratically at 0.9 mL/min. Limit of detection and the limit of quantification were 0.03 and 0.10 µmol/L, respectively. The method exhibited linearity in a concentration range of 0.16 and 5.08 µmol/L. Mean recovery was 106.66%, whereas intra- and interassay precisions were determined to be within 6 RSD%. On average, ERT concentration in different commercially available classes of cow milk was found to be 0.442 ± 0.191 µmol/L, with the highest levels in the ultra-high temperature milks and low values in the unprocessed and HTST whole milks. In this light, our experiments suggest that ERT could be used as a marker for the heat treatment of milk.
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