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A Protocol for microbiologically safe preparation, storage, and use of autologous serum eye-drops in low-income countries

Blasetti, Francesco and Usai, Donatella and Sotgia, Salvatore and Carru, Ciriaco and Zanetti, Stefania Anna Lucia and Pinna, Antonio <1963- > (2015) A Protocol for microbiologically safe preparation, storage, and use of autologous serum eye-drops in low-income countries. The Journal of Infection in Developing Countries, Vol. 9 (1), 055-059. ISSN 2036-6590. eISSN 1972-2680. Article.

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DOI: 10.3855/jidc.4733

Abstract

Introduction: The study aimed to investigate whether the preparation, storage, and use of autologous serum in insulin syringes is microbiologically safe.
Methodology: Blood samples (10 mL) were obtained from 10 volunteers. After centrifugation, the supernatant serum was removed and distributed in 5 sterile insulin syringes for each sample; syringes were numbered 0 to 4 and labelled with the subject’s details. Syringes were immediately transported to the microbiology laboratory and stored in a refrigerator at +4°C. The “0” labelled syringes were separated from the others and 100 μl of serum from each syringe was immediately seeded on chocolate and Sabouraud agar plates, which were incubated aerobically at 37°C for 96 hours to detect any bacterial and/or fungal contamination. In the next 4 days, the same procedure was repeated for the remaining syringes: on day 1, the “1” labelled syringes were analyzed; on day 2, the “2” labelled ones, and so on. In a second experiment, blood samples were obtained from 5 different volunteers. The same procedure as above was followed, but each syringe was used for repeated cultures at 2-hour intervals, for a total of 12 cultures/day. The needle was removed and replaced for each inoculation and the syringes were stored in the refrigerator after use.
Results: Under these experimental conditions, none of the cultures showed microbial growth.
Conclusions: Results suggest that, under the protocol described, preparation, storage and use of undiluted autologous serum in insulin syringes is inexpensive, fast, and microbiologically safe. This is of great importance for low-income countries.

Item Type:Article
ID Code:10777
Status:Published
Refereed:Yes
Uncontrolled Keywords:Autologous serum, preparation, storage, administration, microbiological investigation
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
002 Altri enti e centri di ricerca del Nord Sardegna > AOU -Azienda Ospedaliero Universitaria, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:OLoeP
ISSN:2036-6590
eISSN:1972-2680
Copyright Holders:© 2015 Blasetti et al.
Deposited On:09 Feb 2015 16:38

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