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Evaluation of a nonradiometric system (BACTEC 9000 MB) for detection of mycobacteria in human clinical samples

Zanetti, Stefania Anna Lucia and Ardito, Fausta and Sechi, Leonardo Antonio and Sanguinetti, Maurizio and Molicotti, Paola and Delogu, Giovanni and Pinna, Maria Piera and Nacci, A. and Fadda, Giovanni (1997) Evaluation of a nonradiometric system (BACTEC 9000 MB) for detection of mycobacteria in human clinical samples. Journal of Clinical Microbiology, Vol. 35 (8), p. 2072-2075. ISSN 0095-1137. eISSN 1098-660X. Article.

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Abstract

This study was carried out to evaluate the rate of recovery and time required for detection of mycobacteria from pulmonary and extrapulmonary human clinical samples, by using a fluorescence-quenching-based oxygen sensor (BACTEC 9000 MB; Becton Dickinson Microbiology Systems, Sparks, Md.). The results were compared with those obtained by microscopy, conventional culture in Lowenstein-Jensen (LJ) medium, and a BACTEC radiometric system (BACTEC 460 TB; Becton Dickinson). Of the 779 clinical samples processed, 364 from pulmonary sites and 415 from extrapulmonary sites, 62 (7.9%) were positive for mycobacterial isolates; of the positive samples, 59 (95.1%) were detected with the fluorescent BACTEC 9000 MB system, 57 (91.9%) were detected with the radiometric system (BACTEC 460 TB), and 43 (69.3%) were detected with LJ conventional culture. The mean times to detection of all mycobacteria with BACTEC 9000 MB and BACTEC 460 TB were similar (10.3 and 10.0 days, respectively). The results obtained indicate that the nonradiometric BACTEC (BACTEC 9000 MB) system is as efficient as Bactec 460 TB and significantly more efficient than LJ for the rapid recovery of mycobacteria from both pulmonary and extrapulmonary clinical specimens. Though the BACTEC 9000 MB system is recommended for respiratory specimens, we demonstrated that it can be successfully used also for recovery of mycobacteria from clinical specimens from various extrapulmonary sites.

Item Type:Article
ID Code:10768
Status:Published
Refereed:Yes
Uncontrolled Keywords:Nonradiometric System (BACTEC 9000 MB), mycobacteria, human clinical samples
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
ISSN:0095-1137
eISSN:1098-660X
Deposited On:06 Feb 2015 12:41

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