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Oocyte cryopreservation: oocyte assessment and strategies for improving survival

Ledda, Sergio and Bogliolo, Luisa and Succu, Sara and Ariu, Federica and Bebbere, Daniela and Leoni, Giovanni Giuseppe and Naitana, Salvatore (2006) Oocyte cryopreservation: oocyte assessment and strategies for improving survival. Reproduction, Fertility and Development, Vol. 19 (1), p. 13-23. ISSN 1031-3613. Article.

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DOI: 10.1071/RD06126

Abstract

Despite significant progress in cryopreservation of mammalian oocytes and embryos, many of the molecular and biochemical events that underlie this technology are poorly understood. In recent years, researchers have focused on obtaining viable oocytes that are developmentally competent. Even under the most favourable conditions, experimental approaches have achieved only limited success compared with fresh oocytes used in routine in vitro embryo production. Chilling injuries and toxic effects of the cryoprotectants are the major adverse consequences following cryoprocedures. To overcome these problems, different strategies have been developed for improving cryopreservation results. These strategies include reducing container volumes, increasing the thermal gradient, changing the cell surface/volume ratio, enhancing cryotolerance by supplementation with various additives or modifying the lipid composition of the oocyte membrane. In order to develop new strategies for reducing the various forms of stress associated with oocyte cryopreservation, it is fundamental to gain a better understanding of the major changes responsible for poor post-thaw survival. With this knowledge, we hope that oocyte cryostorage will become a fully reliable reproductive technique in the near future.

Item Type:Article
ID Code:10756
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mammalian oocytes, cryopreservation, experimental approaches
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:CSIRO
ISSN:1031-3613
Deposited On:04 Feb 2015 17:07

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