Solinas, Cinzia and Varcasia, Antonio and Brianti, Emanuele and Giannetto, Salvatore and Pipia, Anna Paola and Columbano, Nicolò and Tosciri, Gabriele and Dantas-Torres, Filipe and Garippa, Giovanni and Otranto, Domenico and Scala, Antonio (2014) Cercopithifilariaspp. in dogs in Sardinia Island (Italy). Parasitology Research, Vol. 113 (2), p. 675-679. ISSN 0932-0113. eISSN 1432-1955. Article.
Full text not available from this repository.
A survey on Cercopithifilaria spp. was carried out on owned and kennelled dogs in Sardinia, Italy. A total of 180 dogs were sampled and tested by microscopic detection or PCR of dermal microfilariae in skin snip sediments. The overall prevalence for Cercopithifilaria spp. at both microscopy and molecular tests was 9.4 % (17/180), while 8.3 % (15/180) of dogs scored positive at microscopic detection of sediments only. Of the 225 microfilariae measured, 212 were identified as Cercopithifilaria bainae and the remaining as Cercopithifilaria sp. II. All samples were molecularly processed for specific amplification of cytochrome oxidase subunit 1 (cox1) and ribosomal 12S gene fragments. The Basic Local Alignment Search Tool analysis of the cox1 and 12S sequences here obtained showed a high nucleotide similarity (99 and 100 %, respectively) with those of C. Bainae available in GenBank. In particular, cox1 haplotype I (HI; n= 14), haplotype HXVIII (n= 2), and a new haplotype, named HXIX (n= 1), differing for a single polymorphism from HI, were detected. This study reports data on the occurrence, distribution, and genetic makeup of C. Bainae and Cercopithifilaria sp. II infesting dogs in Sardinia, suggesting that these filarioids are spread in areas where Rhipicephalus sanguineus sensu lato ticks occur.
I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore
Repository Staff Only: item control page